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Mass spectrometry (MS) datasets of PURPL RNA pulldown samples in A375 cells

ABSTRACT: We performed PURPL RNA pulldown followed by MS analysis to identify purpl-interacting proteins in A375 cells.With high performance liquid chromatography-mass spectrometry (HPLC-MS), the binding protein of PURPL from RNA pull down was detected by removing non-specific protein which were binding with EGFP.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte, Cell Culture

SUBMITTER: xiaoting Liang

LAB HEAD: Liang Xiaoting

PROVIDER: PXD028863 | Pride | 2022-02-16

REPOSITORIES: Pride

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PURPL represses autophagic cell death to promote cutaneous melanoma by modulating ULK1 phosphorylation.

Han Shuo S Li Xue X Wang Ke K Zhu Dingheng D Meng Bingyao B Liu Jieyu J Liang Xiaoting X Jin Yi Y Liu Xingyuan X Wen Qian Q Zhou Liang L

Cell death & disease 20211110 11

Uncontrolled overactivation of autophagy may lead to autophagic cell death, suppression of which is a pro-survival strategy for tumors. However, mechanisms involving key regulators in modulating autophagic cell death remain poorly defined. Here, we report a novel long noncoding RNA, p53 upregulated regulator of p53 levels (PURPL), functions as an oncogene to promote cell proliferation, colony formation, migration, invasiveness, and inhibits cell death in melanoma cells. Mechanistic studies showe ...[more]

PMID: 34759263

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