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Quantitative proteomic analysis of proteins bound to rs452384-T or rs452384-C

ABSTRACT: This project focuses on the functional characterization of a risk variant (SNP) implicated in the predisposition to uveal melanoma, rs452384. We sought to determine whether some nuclear factors could bind with allele-specificity a DNA sequence centered around rs452384 to regulate gene expression. We sought to identify by quantitative mass spectrometry the nuclear proteins bound to double-stranded DNA probes with identical DNA sequences except for rs452384-C or T alleles in the center of the probe. The goal was to quantify the binding of transcription factors to the DNA probes, and to select those enriched (or specific) to the C or T allele of rs452384. To identify proteins specifically bound to rs452384, we compared binding partners to those obtained with a negative control probe known not to recognize any transcription factor.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Uveal Melanoma Cell

DISEASE(S): Uveal Melanoma

SUBMITTER: Victor Laigle

LAB HEAD: Damarys Loew

PROVIDER: PXD028954 | Pride | 2022-11-15

REPOSITORIES: Pride

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