Proteomics

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Secretome profiling of Cryptosporidium sporozoites in the initial interaction processes with host epithelial cells

ABSTRACT: Sporozoites secrete proteins which can help them adhere to/invade host cells during gliding motility on the surface of host cells. Currently, the composition of secreted proteins of C. parvum during adhesion/invasion of host cells is not fully understood. In this study, TMT labeled quantitative proteomics was used for the first time to compare and analyze the secreted proteins of C. parvum in the gliding motility state (3 hpi) and adhesion/invasion state (6 hpi). Bioinformatics analysis of the quantified differentially secreted proteins from C. parvum and HCT-8 cells showed that a total of 626 and 2339 C. parvum and HCT-8 cells secreted proteins were quantified. Differentially secreted proteins of C. parvum are mainly modified after translation, which are related to protein degradation and kinase activity, and cap-domain proteins are highly expressed during sporozoite gliding motility. However, differentially secreted proteins of HCT-8 cells are mainly concentrated in the pathways related to extracellular matrix and cell adhesion molecules. This study identified some important molecules that may be involved in the interaction between C. parvum and HCT-8 cells, providing basic data for the screening of key functional genes and the study of invasion mechanism of C. parvum.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Limnobaculum Parvum

TISSUE(S): Cell Culture

SUBMITTER: Zhaohui Cui  

LAB HEAD: Zhaohui Cui

PROVIDER: PXD028969 | Pride | 2025-11-17

REPOSITORIES: Pride

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Publications

Secretome and Transcriptome Analyses Reveal New Potential Virulence Factors during <i>Cryptosporidium parvum</i> Invasion.

Li Na N   Cui Zhaohui Z   Wang Luyang L   Sun Songying S   Zhao Wenchao W   Wang Yuexin Y   Meng Qingda Q   Li Xiaoying X   Zhang Sumei S   Wu Yayun Y   Zhang Longxian L  

Journal of proteome research 20251025 11

<i>Cryptosporidium parvum</i> is a major cause of life-threatening diarrheal disease in young children and immunocompromised individuals. During the initial infection, <i>C. parvum</i> secretes a large number of virulence proteins that help the parasite invade host cells. However, little is known about these secretory proteins (SPs). Here, we mapped <i>C. parvum</i> SPs during early invasion (3 and 6 h postinfection) by secretomics analyses. A total of 396 SPs were identified. The cgd1_330 was i  ...[more]

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