LC-MS/MS interactomics analysis of Praf2 with timsTOF Pro
Ontology highlight
ABSTRACT:
INSTRUMENT(S): timsTOF Pro
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: 
Chiara guerrera
LAB HEAD: Chiara Guerrera
PROVIDER: PXD029019 | Pride | 2023-12-20
REPOSITORIES: Pride
Publications
Pharmacological chaperone-rescued cystic fibrosis CFTR-F508del mutant overcomes PRAF2-gated access to endoplasmic reticulum exit sites.
Saha Kusumika K Chevalier Benoit B Doly Stéphane S Baatallah Nesrine N Guilbert Thomas T Pranke Iwona I Scott Mark G H MGH Enslen Hervé H Guerrera Chiara C Chuon Cérina C Edelman Aleksander A Sermet-Gaudelus Isabelle I Hinzpeter Alexandre A Marullo Stefano S
Cellular and molecular life sciences : CMLS 20220927 10
The endoplasmic reticulum exit of some polytopic plasma membrane proteins (PMPs) is controlled by arginin-based retention motifs. PRAF2, a gatekeeper which recognizes these motifs, was shown to retain the GABA<sub>B</sub>-receptor GB1 subunit in the ER. We report that PRAF2 can interact on a stoichiometric basis with both wild type and mutant F508del Cystic Fibrosis (CF) Transmembrane Conductance Regulator (CFTR), preventing the access of newly synthesized cargo to ER exit sites. Because of its ...[more]
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