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Identifying the N-terminus of SLX4Nter in HeLa KO30 cells

ABSTRACT: The project was aimed at identifying the N-terminus of a truncated form of SLX4 (termed SLX4Nter) in HeLa KO30 cells. The HeLa KO30 cell line comes from a clone of HeLa Flp-In T-Rex cells (termed FITo) obtained through a CRISPR-Cas9 approach using commercially available plasmids from Santa Cruz Biotechnology (sc-404395 and sc-404395-HDR). This allows the integration of an exogenous plasmid conferring Puromycin resistance at the endogenous SLX4 locus. Unexpectedly, this strategy allowed us to retrieve several Puromycin-resistant clones (including KO30) displaying a shorter form of endogenous SLX4 that is N-terminally truncated (SLX4Nter). To identify the proximal peptide of SLX4Nter, FITo and KO30 nuclear extracts were immunoprecipitated with anti-SLX4 antibodies and N-terminus of SLX4Nter was further identified by LC-MSMS analysis.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: AUDEBERT Stephane

LAB HEAD: Pierre-Henri Gaillard

PROVIDER: PXD029062 | Pride | 2022-02-16

REPOSITORIES: Pride

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