Searching for interactors of endogenous SLX4

ABSTRACT: The project was aimed at identifying binding partners of endogenous SLX4 in HeLa cells. Whole cell extracts from HeLa Flp-In T-Rex cells were used for immunoprecipitation performed with anti-SLX4 antibodies from Bethyl Laboratories (A302-270A and A302-269A), either used alone or in combination. To discriminate bona fide SLX4 partners from non-specific proteins recognized by the anti-SLX4 antibodies, cells were transfected with control (Luc) siRNA or a siRNA targeting SLX4. After immunoprecipitation, protein complexes were analysed by LC-MSMS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: AUDEBERT Stephane  

LAB HEAD: Pierre-Henri Gaillard

PROVIDER: PXD029084 | Pride | 2022-02-16

REPOSITORIES: Pride