Improving the proteome coverage of Daphnia magna

ABSTRACT: To improve proteome coverage in Daphnia, we established an easy-to-use procedure based on the LC-MS/MS of whole daphnids and dissected daphnia guts separately. Using a comprehensive spectral library, generated by gas-phase fractionation (GPF) and a data-independent acquisition method, we identified in total > 6000 proteins, demonstrating the effectiveness of our approach.

INSTRUMENT(S):

ORGANISM(S): Daphnia Magna Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Gut, Whole Body

DISEASE(S): Duchenne Muscular Dystrophy

SUBMITTER: Thomas Fröhlich  

LAB HEAD: Thomas Fröhlich

PROVIDER: PXD029198 | Pride | 2022-11-17

REPOSITORIES: Pride