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Identification of the cell cycle specific proximal interactors of PCDH7

ABSTRACT: We used proximity dependent biotin identification (BioID) method to determine cell cycle specific interaction partners of PCDH7. HeLa S3 cells were synchronized to interphase and mitosis using double tymidine block and double thymidine block followed by S-Trityl-L-cysteine (STC) treatment respectively. Candidate interactors were isolated using streptavidin affinity purification and identified using LC-MS/MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Altuğ Kamacıoğlu

LAB HEAD: Nurhan Ozlu

PROVIDER: PXD029246 | Pride | 2023-02-27

REPOSITORIES: Pride

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Cell cycle-dependent palmitoylation of protocadherin 7 by ZDHHC5 promotes successful cytokinesis.

Ozkan Nazlı Ezgi NE Yigit Berfu Nur BN Degirmenci Beste Senem BS Qureshi Mohammad Haroon MH Yapici Gamze Nur GN Kamacıoglu Altuğ A Bavili Nima N Kiraz Alper A Ozlu Nurhan N

Journal of cell science 20230320 6

Cell division requires dramatic reorganization of the cell cortex, which is primarily driven by the actomyosin network. We previously reported that protocadherin 7 (PCDH7) gets enriched at the cell surface during mitosis, which is required to build up the full mitotic rounding pressure. Here, we report that PCDH7 interacts with and is palmitoylated by the palmitoyltransferase, ZDHHC5. PCDH7 and ZDHHC5 colocalize at the mitotic cell surface and translocate to the cleavage furrow during cytokinesi ...[more]

PMID: 36762613

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