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Dietary sugar shifts mitochondrial metabolism and small RNA biogenesis in sperm

ABSTRACT: It is known that exogenous factors influences sperm quality and function, such as toxins, exercise, diet and smoking. Still, the moleuclar mechanisms of this is not understood. Here, we set out to determine how a dietary insult of different sugar levels in the diet affects Drosophila sperm proteome, by dissecting out seminal vesicles containing mature sperm from adult flies. These flies have been on a dietary intervention of two days on a sugar diet of 3, 30 or 300 g/L of sugar. Whole seminal vesicles were then used for mass spectometry to increase our understanding of how an acute dietary intervention may change sperm proteomics.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Sperm, Epithelial Cell, Seminal Vesicle

SUBMITTER: Signe Skog

LAB HEAD: Anita Öst

PROVIDER: PXD029265 | Pride | 2023-07-20

REPOSITORIES: Pride

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Dietary Sugar Shifts Mitochondrial Metabolism and Small RNA Biogenesis in Sperm.

Ramesh Rashmi R Skog Signe S Örkenby Lovisa L Kugelberg Unn U Nätt Daniel D Öst Anita A

Antioxidants & redox signaling 20230223 16-18

Aims: Increasing concentrations of dietary sugar results in a linear accumulation of triglycerides in male Drosophila, while inducing a U-shaped obesity response in their offspring. Here, using a combination of proteomics and small RNA (sRNA) sequencing, we aimed at understanding the molecular underpinning in sperm for such plasticity. Results: Proteomic analysis of seminal vesicles revealed that increasing concentrations of dietary sugar resulted in a bell-sha ...[more]

PMID: 36509450

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