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Proteomics of liver from mice fed high fat diet (HFD) or regular chow diet

ABSTRACT: Proteomics of liver tissue from mice fed a high fat diet (HFD) or regular chow diet. Data accompany our paper entitled “Dynamic Regulation of N6,2′-O-dimethyladenosine (m6Am) in Obesity” scheduled for publication in Nature Communications, 2021

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Moshe Shay Ben-Haim

LAB HEAD: Gideon Rechavi

PROVIDER: PXD029280 | Pride | 2021-10-27

REPOSITORIES: Pride

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Dynamic regulation of N6,2'-O-dimethyladenosine (m6Am) in obesity.

Ben-Haim Moshe Shay MS Pinto Yishay Y Moshitch-Moshkovitz Sharon S Hershkovitz Vera V Kol Nitzan N Diamant-Levi Tammy T Beeri Michal Schnaider MS Amariglio Ninette N Cohen Haim Y HY Rechavi Gideon G

Nature communications 20211210 1

The prevalent m6Am mRNA cap modification was recently identified as a valid target for removal by the human obesity gene FTO along with the previously established m6A mRNA modification. However, the deposition and dynamics of m6Am in regulating obesity are unknown. Here, we investigate the liver m6A/m methylomes in mice fed on a high fat Western-diet and in ob/ob mice. We find that FTO levels are elevated in fat mice, and that genes which lost m6< ...[more]

PMID: 34893620

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Proteomics of HEPG2 cells following FTO overexpression and knockdown

Project description:Proteomics of HEPG2 cells following FTO overexpression and knockdown. Data accompany our paper entitled “Dynamic Regulation of N6,2′-O-dimethyladenosine (m6Am) in Obesity” scheduled for publication in Nature Communications, 2021

2021-10-27 | PXD029268 | Pride

Proteomics of liver from ob/ob and WT mice

Project description:Proteomics of liver tissue from ob/ob and WT mice. Data accompany our paper entitled “Dynamic Regulation of N6,2′-O-dimethyladenosine (m6Am) in Obesity” scheduled for publication in Nature Communications, 2021

2021-10-27 | PXD029217 | Pride

m6A/m-Seq of mouse adult cortex of Mettl3 cKO or Fto cKO mice

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2018-07-10 | GSE113793 | GEO

Reversible methylation of m6Am in the 5′ cap controls mRNA stability

Project description:Internal bases in mRNA can be subjected to modifications that influence the fate of mRNA in cells. One of the most prevalent modified bases is found at the 5′ end of mRNA, at the first encoded nucleotide adjacent to the 7-methylguanosine cap. Here we show that this nucleotide, N6,2′-O-dimethyladenosine (m6Am), is a reversible modification that influences cellular mRNA fate. Using a transcriptome-wide map of m6Am we find that m6Am-initiated transcripts are markedly more stable than mRNAs that begin with other nucleotides. We show that the enhanced stability of m6Am-initiated transcripts is due to resistance to the mRNA-decapping enzyme DCP2. Moreover, we find that m6Am is selectively demethylated by fat mass and obesity-associated protein (FTO). FTO preferentially demethylates m6Am rather than N6-methyladenosine (m6A), and reduces the stability of m6Am mRNAs. Together, these findings show that the methylation status of m6Am in the 5′ cap is a dynamic and reversible epitranscriptomic modification that determines mRNA stability.

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2018-07-10 | GSE113796 | GEO

Identification of the m6Am Methyltransferase PCIF1 Reveals the Location and Functions of m6Am in the Transcriptome

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2018-07-10 | GSE113789 | GEO

m6A/m-Seq of mouse adult cortex after acute stress

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2018-07-10 | GSE113781 | GEO

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