Dataset Information

Proteomics analysis on cottonseed meal protein isolate extract-ed using improved gossypol removal method

ABSTRACT: Cottonseed meal (CSM) contains a high amount of underutilised proteins due to the presence of gossypol, a toxic compound unsuitable for human consumption. We designed a protein extract process to generate CSM protein isolate (CSMPI) with ultra-low gossypol content to unlock their potential. This process utilised alkaline-based protein extraction with gossypol removal through a multistep acidified organic solvent treatment. Overall, our method successfully yielded 22% of CSMPI from CSM and achieved a protein purity of 92.6%. The method has reduced the free and total gossypol content of the post-treated CSMPI to 4.8ppm and 147.2ppm, respectively, which is much lower than the limit set by US FDA. Proteomics analysis indicated that the gossypol re-moval treatment increased protein identification and enhanced the nutritional quality of the CSMPI due to increased branched-chain amino acids (BCAAs) content after treatment. Overall, our results help to navigate and direct the application of CSMPI towards nutritive human food application.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Gossypium Hirsutum

SUBMITTER: Soon Hong Kwan

LAB HEAD: Bi Xuezhi

PROVIDER: PXD029330 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective.

Tan Chee Fan CF Kwan Soon Hong SH Lee Chun Shing CS Soh Yan Ni Annie YNA Ho Ying Swan YS Bi Xuezhi X

International journal of molecular sciences 20220903 17

Cottonseed meal (CSM) is a good source of dietary proteins but is unsuitable for human consumption due to its gossypol content. To unlock its potential, we developed a protein extraction process with a gossypol removal treatment to generate CSM protein isolate (CSMPI) with ultra-low gossypol content. This process successfully reduced the free and total gossypol content to 4.8 ppm and 147.2 ppm, respectively, far below the US FDA limit. In addition, the functional characterisation of CSMPI reveal ...[more]

PMID: 36077502

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Project description:External application of acetic acid has been recently reported to enhance the survival to drought in plants such as Arabidopsis, rapeseed, maize, rice and wheat, but the effects of acetic acid application on increased drought tolerance in woody plants such as a tropical crop “cassava” remain elusive. A molecular understanding of acetic acid-induced drought avoidance in cassava will contribute to the development of technology that can be used to enhance drought tolerance without resorting to transgenic technology or advancements in cassava cultivation. In the present study, morphological, physiological and molecular responses to drought were analyzed in cassava after the treatment with acetic acid. Results indicated that the acetic acid-treated cassava plants had a higher level of drought avoidance than water-treated, control plants. Specifically, higher leaf relative water content, and chlorophyll and carotenoid levels were observed as soils dried out during the drought treatment. Leaf temperatures in acetic acid-treated cassava plants were higher relative to leaves on plants pretreated with water and the increase of ABA content was observed in leaves of acetic acid-treated plants, suggesting that stomatal conductance and the transpiration rate in leaves of acetic acid-treated plants decreased to maintain relative water contents and avoid drought. Transcriptome analysis revealed that the acetic acid treatment increased the expression of ABA signaling-related genes, such as OPEN STOMATA 1　(OST1) and protein phosphatase 2C; as well as drought response and tolerance-related genes, such as outer membrane tryptophan-rich sensory protein (TSPO), and heat shock proteins. Collectively, the external application of acetic acid enhances drought avoidance in cassava through the upregulation of ABA signaling pathway genes and several stress response- and tolerance-related genes. These data support the idea that adjustments of the acetic acid application to plants is useful to enhance drought tolerance in order to minimize the growth inhibition in the agricultural field.

Dataset Information

Proteomics analysis on cottonseed meal protein isolate extract-ed using improved gossypol removal method

Publications

Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective.

Similar Datasets

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