Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HMADS cells secretome identification LC/MS/MS

ABSTRACT: The aim of the project was to identify secreted proteins by human multipotent adipose derived stem cells differentiated into white and brown adipocytes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Subcutaneous Adipose Tissue

SUBMITTER: Adhideb Ghosh

LAB HEAD: Prof. Dr. Christian Wolfrum

PROVIDER: PXD029335 | Pride | 2021-12-01

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20150826mb_7611_b_002.raw	Raw
	20150826mb_7611_w.raw	Raw
	F223158_20150826mb_7611_w.dat	Other
	F223212_20150826mb_7611_b_002.dat	Other
	checksum.txt	Txt

Items per page:

1 - 5 of 5

Publications

GPR180 is a component of TGFβ signalling that promotes thermogenic adipocyte function and mediates the metabolic effects of the adipocyte-secreted factor CTHRC1.

Balazova Lucia L Balaz Miroslav M Horvath Carla C Horváth Áron Á Moser Caroline C Kovanicova Zuzana Z Ghosh Adhideb A Ghoshdastider Umesh U Efthymiou Vissarion V Kiehlmann Elke E Sun Wenfei W Dong Hua H Ding Lianggong L Amri Ez-Zoubir EZ Nuutila Pirjo P Virtanen Kirsi A KA Niemi Tarja T Ukropcova Barbara B Ukropec Jozef J Pelczar Pawel P Lamla Thorsten T Hamilton Bradford B Neubauer Heike H Wolfrum Christian C

Nature communications 20211208 1

Activation of thermogenic brown and beige adipocytes is considered as a strategy to improve metabolic control. Here, we identify GPR180 as a receptor regulating brown and beige adipocyte function and whole-body glucose homeostasis, whose expression in humans is associated with improved metabolic control. We demonstrate that GPR180 is not a GPCR but a component of the TGFβ signalling pathway and regulates the activity of the TGFβ receptor complex through SMAD3 phosphorylation. In addition, using ...[more]

PMID: 34880217

Similar Datasets

Impaired adipose-neuronal crosstalk in obesity

Project description:Label-free LC-MS/MS was used to characterise and quantify the secretory profiles of murine perivascular adipose tissue, canonical white adipose tissue, and brown adipose tissue.

2022-11-15 | PXD031271 | Pride

Cancer cell-derived ADAM15 dictates macrophage infiltration in colorectal tumors

Project description:Tumor progression and response to treatment is highly affected by interactions between cancer cells and the tumor microenvironment (TME). Many of the soluble factors and signaling receptors involved in this crosstalk are shed by a disintegrin and metalloproteinases (ADAMs). Upregulation of ADAM15 has been linked to worse survival in cancer patients and a tumor-promoting function both in vitro and in murine cancer models. Although ADAM15 has been involved in cell-cell and cell-extracellular matrix interactions, its role in the crosstalk between cancer cells and the TME in vivo remains unexplored. Therefore, we aimed to understand how ADAM15 regulates the cell composition of the TME and how it affects tumor progression. Here, we showed an upregulation of ADAM15 in tumor tissues from rectal cancer patients. Subcutaneous injection of wildtype and ADAM15-knockout CT26 colon cancer cells in syngeneic mice confirmed the pro-tumorigenic role of ADAM15. Profiling of tumors revealed higher immune cell infiltration and cancer cell apoptosis in the ADAM15-deficient tumors. Specifically, loss of ADAM15 led to a reduced number of granulocytes and higher infiltration of antigen-presenting cells, including dendritic cells and macrophages, as well as more T cells. Using in vitro assays, we confirmed the regulatory effect of ADAM15 on macrophage migration and identified ADAM15-derived CYR61 as a potential molecular mediator of this effect. Together, our data suggest that targeting ADAM15 could increase the infiltration of immune cells in colorectal tumors and thereby turn cold tumors hot, resulting in a potentially improved response to combined immunotherapy.

2023-07-24 | PXD041164 | Pride

PPARa effects on human brite adipocyte formation

Project description:Human multipotent adipose-derived stem (hMADS) cells are differentiated in white or brown adipocytes with Rosiglitazone between days 14 and 18. PPAR alpha was silenced by siRNA transfections at day 10.

2023-09-30 | E-MTAB-12937 | biostudies-arrayexpress

microRNA expression in C3H10T1/2 cells treated with BMP7 vs vehicle

Project description:Multipotent C3H10T1/2 cells can be induced to differentiate into mature brown adipocytes by 3-days BMP7 pretreatment followed by standard adipogenic induction.

2015-07-22 | GSE71157 | GEO

Mesenchymal Stromal Cells: What are the secrets of their secretome?

Project description:Multipotent Mesenchymal Stromal Cells (MSCs) are multipotent adult cells that can be isolated from different tissues. The current hypothesis supports that the main MSCs action mechanism relates to its paracrine activity, creating a microenvironment with trophic signals. However, preclinical studies with different sources of MSCs and different animal models have shown conflicting results. Therefore, the evaluation of these cells secretome content is of great interest. Here we analyzed the secreted proteins of MSCs, isolated from 3 different sources (adipose tissue, uterine tubes and skeletal muscle), obtained from 5 different donors. Following MSCs characterization, proteins secreted in serum-free conditions (conditioned media, CM) were analyzed by mass-spectrometry-based quantitative proteomics.

2016-12-09 | PXD003978 | Pride

Control of Embryonic Brown Adipocyte Development by GATA6 I

2023-07-19 | GSE214345 | GEO

Control of Embryonic Brown Adipocyte Development by GATA6 II

2023-07-19 | GSE233954 | GEO

Control of Embryonic Brown Adipocyte Development by GATA6 [ChIP-Seq]

Project description:Brown adipose tissue (BAT) is a thermogenic organ that protects animals against hypothermia and obesity. BAT derives from the multipotent paraxial mesoderm; however, the identity of embryonic brown fat progenitor cells and regulators of adipogenic commitment are unclear. We identified the transcription factor GATA6 as a selective marker of brown adipogenic progenitor cells. Deletion of Gata6 in the brown fat lineage resulted in a striking loss of BAT. To gain insight into the mechanism by which GATA6 supports BAT development, we performed ChIP-seq for GATA6 from the BAT of embryonic day 15.5 embryos.

2023-07-19 | GSE234012 | GEO

Comparative secretome analysis of primary murine white and brown adipocytes

Project description:The adipose organ, including white and brown adipose tissues, is an important player in systemic energy homeostasis, storing excess energy in form of lipids while releasing energy upon various energy demands. Recent studies have demonstrated that white and brown adipocytes also function as endocrine cells and regulate systemic metabolism by secreting factors that act locally and systemically. However, a comparative proteomic analysis of secreted factors from white and brown adipocytes and their responsiveness to adrenergic stimulation has not been reported yet. Therefore, we studied and compared the secretome of white and brown adipocytes, with and without norepinephrine (NE) stimulation. Our results reveal that in the absence of NE, carbohydrate metabolism-regulating proteins are preferably secreted from white adipocytes, while brown adipocytes predominantly secrete integrin signaling proteins. Upon NE stimulation, white adipocytes secrete more proteins involved in lipid metabolism, while brown adipocytes secrete more proteins with specific anti-inflammatory properties. In conclusion, our study provides a comprehensive catalogue of novel adipokine candidates secreted from white and brown adipocytes with many of them responsive to NE.

2018-09-04 | PXD009280 | Pride

Expression data from C3H10T1/2 cells treated with 3.3 nM BMP7 vs vehicle

Project description:Multipotent C3H10T1/2 cells can be induced to differentiate into mature brown adipocytes by 3-days BMP7 pretreatment followed by standard adipogenic induction. We used microarrays to detail the global programme of gene expression in C3H10T1/2 cells after 3-day BMP7 (3.3 nM) treatment.

2015-07-21 | GSE71101 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data