Dataset Information

HMADS cells secretome identification LC/MS/MS

ABSTRACT: The aim of the project was to identify secreted proteins by human multipotent adipose derived stem cells differentiated into white and brown adipocytes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Subcutaneous Adipose Tissue

SUBMITTER: Adhideb Ghosh

LAB HEAD: Prof. Dr. Christian Wolfrum

PROVIDER: PXD029335 | Pride | 2021-12-01

REPOSITORIES: Pride

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Publications

GPR180 is a component of TGFβ signalling that promotes thermogenic adipocyte function and mediates the metabolic effects of the adipocyte-secreted factor CTHRC1.

Balazova Lucia L Balaz Miroslav M Horvath Carla C Horváth Áron Á Moser Caroline C Kovanicova Zuzana Z Ghosh Adhideb A Ghoshdastider Umesh U Efthymiou Vissarion V Kiehlmann Elke E Sun Wenfei W Dong Hua H Ding Lianggong L Amri Ez-Zoubir EZ Nuutila Pirjo P Virtanen Kirsi A KA Niemi Tarja T Ukropcova Barbara B Ukropec Jozef J Pelczar Pawel P Lamla Thorsten T Hamilton Bradford B Neubauer Heike H Wolfrum Christian C

Nature communications 20211208 1

Activation of thermogenic brown and beige adipocytes is considered as a strategy to improve metabolic control. Here, we identify GPR180 as a receptor regulating brown and beige adipocyte function and whole-body glucose homeostasis, whose expression in humans is associated with improved metabolic control. We demonstrate that GPR180 is not a GPCR but a component of the TGFβ signalling pathway and regulates the activity of the TGFβ receptor complex through SMAD3 phosphorylation. In addition, using ...[more]

PMID: 34880217

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Project description:Ramirez2017 - Human global metabolism in brown and white adipocytes Recon 2.1A, an update to Recon 2.1x, is suitable for quantitatively-realistic results for flux balance analysis in human metabolism. This model is described in the article: Integrating Extracellular Flux Measurements and Genome-Scale Modeling Reveals Differences between Brown and White Adipocytes. Ramirez AK, Lynes MD, Shamsi F, Xue R, Tseng YH, Kahn CR, Kasif S, Dreyfuss JM. Cell Rep 2017 Dec; 21(11): 3040-3048 Abstract: White adipocytes are specialized for energy storage, whereas brown adipocytes are specialized for energy expenditure. Explicating this difference can help identify therapeutic targets for obesity. A common tool to assess metabolic differences between such cells is the Seahorse Extracellular Flux (XF) Analyzer, which measures oxygen consumption and media acidification in the presence of different substrates and perturbagens. Here, we integrate the Analyzer's metabolic profile from human white and brown adipocytes with a genome-scale metabolic model to predict flux differences across the metabolic map. Predictions matched experimental data for the metabolite 4-aminobutyrate, the protein ABAT, and the fluxes for glucose, glutamine, and palmitate. We also uncovered a difference in how adipocytes dispose of nitrogenous waste, with brown adipocytes secreting less ammonia and more urea than white adipocytes. Thus, the method and software we developed allow for broader metabolic phenotyping and provide a distinct approach to uncovering metabolic differences. This model is hosted on BioModels Database and identified by: MODEL1703310000. To cite BioModels Database, please use: Chelliah V et al. BioModels: ten-year anniversary. Nucl. Acids Res. 2015, 43(Database issue):D542-8. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

HMADS cells secretome identification LC/MS/MS

Publications

GPR180 is a component of TGFβ signalling that promotes thermogenic adipocyte function and mediates the metabolic effects of the adipocyte-secreted factor CTHRC1.

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