Dataset Information

Proteomic profiling of canine fibrosarcomas and adjacent unaffected peritumoral tissue using laser-capture microdissected FFPE tissue

ABSTRACT: Proteomic profiling of canine fibrosarcomas and adjacent unaffected peritumoral tissue using laser-capture microdissected FFPE tissue

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Skeletal Muscle, Ccd-18co Cell, Adipose Tissue, Connective Tissue

DISEASE(S): Fibrosarcoma

SUBMITTER: Laura Kunz

LAB HEAD: Enni Markkanen

PROVIDER: PXD029338 | Pride | 2023-03-11

REPOSITORIES: Pride

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Publications

Proteomic profiling of canine fibrosarcoma and adjacent peritumoral tissue.

Beebe Erin E Pöschel Amiskwia A Kunz Laura L Wolski Witold W Motamed Zahra Z Meier Daniela D Guscetti Franco F Nolff Mirja C MC Markkanen Enni E

Neoplasia (New York, N.Y.) 20221209

Fibrosarcoma (FSA) are rare soft tissue tumors that display aggressive local behavior and invasive growth leading to high rates of tumor recurrence. While the low incidence in humans hampers detailed understanding of the disease, FSA are frequent in dogs and present potential models for the human condition. However, a lack of in-depth molecular characterization of FSA and unaffected peritumoral tissue (PTT) in both species impedes the translational potential of dogs. To address this shortcoming, ...[more]

PMID: 36508875

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Project description:The pollution of the environment with microplastics has been recognized as an emerging threat worldwide. Due to an exponential increase in production of plastic over the last eight decades and its longevity in the environment, accumulating amounts of microplastic are polluting rivers, lakes and the ocean. Their entry pathways are diverse and still only incompletely understood. Since microplastics are usually defined smaller than 5 mm, it can be ingested by a wide range of aquatic organisms including teleost fish. There are different approaches to study the detrimental effects of pollutants on aquatic organisms. On the one hand, generic baseline parameters such as growth and mortality are regularly considered, often accompanied by established stress parameters such as cortisol, heat shock proteins or lipid oxidation. The conflicting findings to date suggest that these parameters might not be sensitive enough to indicate the physiological effects of environmentally relevant microplastic concentrations. For this reason, more sophisticated biological approaches could provide new insights into whether and how microplastics harm fish. To date, proteomic approaches have been used only sporadically when investigating the effects of microplastic exposure on aquatic organisms. So far, this approach has not been used to address potential microplastic impacts in fish. In the present study, a proteomic approach was trialed alongside established methods in an investigation of fish experiencing long-term exposure to environmentally relevant concentrations of microplastics. Two groups of rainbow trout (Oncorhynchus mykiss were exposed to microplastic concentrations and sizes currently encountered in wild fish and an increased concentration, expected to occur in the near future. These groups where compared to a control group maintained in MP free conditions. Five fish of each treatment were sampled at three time points (week 1, week 4, week 17). The experiments were performed in triplicates, resulting in 45 samples used in the proteomic analysis.

Dataset Information

Proteomic profiling of canine fibrosarcomas and adjacent unaffected peritumoral tissue using laser-capture microdissected FFPE tissue

Publications

Proteomic profiling of canine fibrosarcoma and adjacent peritumoral tissue.

Similar Datasets

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