Multiplexed isobaric quantitative cross-linking reveals drug-induced interactome changes in breast cancer cells

ABSTRACT: In vivo application of an extended set of six iqPIR reagents (6-plex iqPIR) allowing multiplexed quantitative interactome analysis of up to six biological samples in a single LC-MS acquisition. Multiplexed iqPIR is demonstrated on MCF-7 breast cancer cells treated with five different Hsp90 inhibitors revealing large scale protein conformational and interaction changes specific to the molecular class of the inhibitors.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Helisa Wippel  

LAB HEAD: James E. Bruce

PROVIDER: PXD029350 | Pride | 2023-03-11

REPOSITORIES: Pride