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Multiplexed isobaric quantitative cross-linking reveals drug-induced interactome changes in breast cancer cells

ABSTRACT: In vivo application of an extended set of six iqPIR reagents (6-plex iqPIR) allowing multiplexed quantitative interactome analysis of up to six biological samples in a single LC-MS acquisition. Multiplexed iqPIR is demonstrated on MCF-7 breast cancer cells treated with five different Hsp90 inhibitors revealing large scale protein conformational and interaction changes specific to the molecular class of the inhibitors.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Helisa Wippel

LAB HEAD: James E. Bruce

PROVIDER: PXD029350 | Pride | 2023-03-11

REPOSITORIES: Pride

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			Action	DRS
	100721_MCF7_6plex_hsp90_SEC_DDA_F1_5_biorep1_1.raw	Raw
	100721_MCF7_6plex_hsp90_SEC_DDA_F1_5_biorep1_2.raw	Raw
	100721_MCF7_6plex_hsp90_SEC_DDA_F1_5_biorep1_3.raw	Raw
	100721_MCF7_6plex_hsp90_SEC_F10_biorep1_1.raw	Raw
	100721_MCF7_6plex_hsp90_SEC_F10_biorep1_2.raw	Raw

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Publications

Multiplexed Isobaric Quantitative Cross-Linking Reveals Drug-Induced Interactome Changes in Breast Cancer Cells.

Wippel Helisa H HH Chavez Juan D JD Keller Andrew D AD Bruce James E JE

Analytical chemistry 20220202 6

The study of protein structures and interactions is critical to understand their function. Chemical cross-linking of proteins with mass spectrometry (XL-MS) is a rapidly developing structural biology technique able to provide valuable insight into protein conformations and interactions, even as they exist within their native cellular environment. Quantitative analysis of cross-links can reveal protein conformational and interaction changes that occur as a result of altered biological states, env ...[more]

PMID: 35107270

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