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Complexome profiling of mitochondria to study organismic consequences of aging in Podospora anserina

ABSTRACT: Podospora anserina is an established aging model with a strong mitochondrial etiology of aging. Here we performed a complexome analysis of isolated mitochondria to study age-related changes of assembled mitochondrial protein complexes. The analysis revealed prominent age-related alterations in oxidative phosphorylation (OXPHOS) and the induction of non-mitochondrial salvage pathways.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Podospora Anserina

SUBMITTER: Ilka Wittig

LAB HEAD: Heinz D. Osiewacz

PROVIDER: PXD029352 | Pride | 2022-02-22

REPOSITORIES: Pride

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Aging of <i>Podospora anserina</i> Leads to Alterations of OXPHOS and the Induction of Non-Mitochondrial Salvage Pathways.

Warnsmann Verena V Meisterknecht Jana J Wittig Ilka I Osiewacz Heinz D HD

Cells 20211126 12

The accumulation of functionally impaired mitochondria is a key event in aging. Previous works with the fungal aging model <i>Podospora anserina</i> demonstrated pronounced age-dependent changes of mitochondrial morphology and ultrastructure, as well as alterations of transcript and protein levels, including individual proteins of the oxidative phosphorylation (OXPHOS). The identified protein changes do not reflect the level of the whole protein complexes as they function in-vivo. In the present ...[more]

PMID: 34943827

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