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Proteomic profiling and alteration in Tuta absoluta upon infection with entomopathogenic fungi, Beauveria bassiana and Purpureocillium lilacinum

ABSTRACT: Tomato leafminer (Tuta absoluta) is a major pest of solanaceous plant species and causes heavy economic losses to the tomato crop. T. absoluta have been developed resistance to many chemical insecticides and have serious impacts on human health as well as environment. To overcome the ill effects of insecticides, ecofriendly approachis needed and among various biological microbes, entomopathogenic fungi is reliable and cost effective. The entomopathogenic fungi, like Beauveria bassiana and Purpureocillium lilacinum have ability to control pest population. The present study was conducted to study the interaction of B. bassiana and P. lilacinum at proteomic level to understand post infection effects. The analysis showed that in fourth instar larvae of T. absoluta, and the 442 and 423 proteins were significantly dysregulated upon infection with P. lilacinum and B. bassiana respectively. The present study concluded entomopathogenic fungal infection decline the immune system of T. absoluta larvae and can be adopted in for control of T. absoluta insect pest.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bombyx Mori (silk Moth)

TISSUE(S): Whole Body

SUBMITTER: Gurmeet Kour Bali

LAB HEAD: Dr. Radhakrisknan S Pandit

PROVIDER: PXD029374 | Pride | 2022-07-05

REPOSITORIES: Pride

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Project description:A genomic insight into how an insect pest responds to the infection of a fungal insect pathogen, such as Beauveria bassiana, is critical for alternative strategy of insect pest contol based on fungal insecticides but has not been well probed. Here we constructed three pairs of digital expression libraries (transcriptomes) of Plutella xylostella (global lepidopteran pest) larvae 24, 36 and 48 hours post treatment of infection (hptI) and control (hptC) to reveal the host response to B. bassiana infection at genomic level. The paired libraries comprised 2144, 3200 and 2967 differentially expressed genes (DEGs) of P. xylostella at 24, 36 and 48 hptI/hptC, respectively. These DEGs were enriched in various immune pathways activated by the fungal infection, such as the pathways of complement and coagulation cascades, protein digestion and absorption, and drug metabolism - cytochrome P450. We found that 24 hptI was critical either for the cuticular penetration of B. bassiana or for the initial activation of the host defense system. The host immune response peaked at 36 hptI so that multiple defense mechanisms were activated against the fungal entry into the host hemocoel. At 48 hptI, many host genes involved in immunity and metabolism were downregulated, suggesting a success of fungal localization in the host hemocoel by overcoming the host defense reaction. Finally, we revealed that several fungal pathways could play important roles in the host-pathogen interaction, such as antioxidant activity, peroxidase activity and proteolysis. Up to 1636 fungal genes were co-expressed at the three time points, and 116 of them encode putative secretion proteins. Our results provide a novel insight into the pathogen-insect interaction and help to probe molecular mechanisms involved in the control of P. xylostella by B. bassiana. Here we constructed three pairs of digital expression libraries (transcriptomes) of Plutella xylostella (global lepidopteran pest) larvae 24, 36 and 48 hours post treatment of infection (hptI) and control (hptC) to reveal the host response to B. bassiana infection at genomic level

Dataset Information

Proteomic profiling and alteration in Tuta absoluta upon infection with entomopathogenic fungi, Beauveria bassiana and Purpureocillium lilacinum

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