Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Early Embryonic Cell
SUBMITTER: Vytautas Iesmantavicius
LAB HEAD: Marc Buehler
PROVIDER: PXD029392 | Pride | 2023-09-07
REPOSITORIES: Pride
Flemr Matyas M Schwaiger Michaela M Hess Daniel D Iesmantavicius Vytautas V Ahel Josip J Tuck Alex Charles AC Mohn Fabio F Bühler Marc M
RNA (New York, N.Y.) 20230503 8
Removal of introns during pre-mRNA splicing, which is central to gene expression, initiates by base pairing of U1 snRNA with a 5' splice site (5'SS). In mammals, many introns contain weak 5'SSs that are not efficiently recognized by the canonical U1 snRNP, suggesting alternative mechanisms exist. Here, we develop a cross-linking immunoprecipitation coupled to a high-throughput sequencing method, BCLIP-seq, to identify NRDE2 (nuclear RNAi-defective 2), and CCDC174 (coiled-coil domain-containing 1 ...[more]