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Mass spectrometry-based characterization of secretomes from different strains of Pseudomonas chlororaphis and Pseudomonas entomophila.

ABSTRACT: To characterize the molecular basis of cytotoxicity of different Pseudomonas species and strains, we analyzed the protein content of secretomes of three P. chlororaphis strains (CIP63, CIP75 and the reference strain PA23) and of six P. entomophila strains (L48 WT, or deleted for various virulence factors: the global activator GacA, the pore-forming toxin Mnl, the pore-forming toxin ExlA, and double mutants for these genes).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Pseudomonas Entomophila L48 Pseudomonas Chlororaphis

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD029397 | Pride | 2022-07-25

REPOSITORIES: Pride

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Genomic erosion and horizontal gene transfer shape functional differences of the ExlA toxin in Pseudomonas spp.

Job Viviana V Gomez-Valero Laura L Renier Adèle A Rusniok Christophe C Bouillot Stephanie S Chenal-Francisque Viviane V Gueguen Erwan E Adrait Annie A Robert-Genthon Mylène M Jeannot Katy K Panchev Peter P Elsen Sylvie S Fauvarque Marie-Odile MO Couté Yohann Y Buchrieser Carmen C Attrée Ina I

iScience 20220614 7

Two-partner secretion (TPS) is widespread in the bacterial world. The pore-forming TPS toxin ExlA of Pseudomonas aeruginosa is conserved in pathogenic and environmental Pseudomonas. While P. chlororaphis and P. entomophila displayed ExlA-dependent killing, P. putida did not cause damage to eukaryotic cells. ExlA proteins interacted with epithelial cell membranes; however, only ExlA Pch induced the cleavage of the adhesive molecule E-cadherin. ...[more]

PMID: 35789842

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