Dataset Information

Proteome and phosphoproteome analysis of autophagy deficient ATG5flox:CamKII-Cre and ATG5flox:VGAT-Cre conditional knockout mice

ABSTRACT: The experiment was performed to find selective targets of autophagy in different subtypes of neurons. Therefore, brain tissue of conditional knockout mice lacking autophagy protein ATG5 in inhibitory or excitatory neurons was used. Cortices (ATG5flox:CamKII-Cre) / striata (ATG5flox:VGAT-Cre) of mice were mixed with lys6 labeled brain lysates as internal controls between the samples. Samples were measured by LC-MS/MS and resulting H/L ratios were used to quantify proteomic and phosphoproteomic changes.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Frederik Tellkamp

LAB HEAD: Natalia L Kononenko

PROVIDER: PXD029435 | Pride | 2022-11-15

REPOSITORIES: Pride

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Autophagy regulates neuronal excitability by controlling cAMP/protein kinase A signaling at the synapse.

Overhoff Melina M Tellkamp Frederik F Hess Simon S Tolve Marianna M Tutas Janine J Faerfers Marcel M Ickert Lotte L Mohammadi Milad M De Bruyckere Elodie E Kallergi Emmanouela E Delle Vedove Andrea A Nikoletopoulou Vassiliki V Wirth Brunhilde B Isensee Joerg J Hucho Tim T Puchkov Dmytro D Isbrandt Dirk D Krueger Marcus M Kloppenburg Peter P Kononenko Natalia L NL

The EMBO journal 20221011 22

Autophagy provides nutrients during starvation and eliminates detrimental cellular components. However, accumulating evidence indicates that autophagy is not merely a housekeeping process. Here, by combining mouse models of neuron-specific ATG5 deficiency in either excitatory or inhibitory neurons with quantitative proteomics, high-content microscopy, and live-imaging approaches, we show that autophagy protein ATG5 functions in neurons to regulate cAMP-dependent protein kinase A (PKA)-mediated p ...[more]

PMID: 36217825

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	MQ_output.7z	Other
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	MaxQuant_1.6.14.0.zip	Other
	Raw_files.7z	Other
	experimentalDesign.xlsx	Xlsx

Dataset Information

Proteome and phosphoproteome analysis of autophagy deficient ATG5flox:CamKII-Cre and ATG5flox:VGAT-Cre conditional knockout mice

Dataset's files

Publications

Autophagy regulates neuronal excitability by controlling cAMP/protein kinase A signaling at the synapse.

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