Proteomics

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Clostridioides difficile phosphoproteomics shows an expansion of phosphorylated proteins in stationary growth phase


ABSTRACT: Phosphorylation is a post-translational modification that can affect both house-keeping functions and virulence characteristics in bacterial pathogens. In the Gram-positive enteropathogen Clostridioides difficile the extent and nature of phosphorylation events is poorly characterized, though a protein-kinase mutant strain demonstrates pleiotropic phenotypes. Here, we used an immobilized metal affinity chromatography strategy to characterize serine, threonine and tyrosine phosphorylation in C. difficile. We find limited protein phosphorylation in the exponential growth phase but a sharp increase in the number of phosphopeptides after the onset of stationary growth phase. Among the overall more than 1500 phosphosites, our approach identifies expected targets and phosphorylation sites, including the protein kinase PrkC, the anti-sigma-F factor antagonist (SpoIIAA), the anti-sigma-B factor antagonist (RsbV) and HPr kinase/phosphorylase (HprK).. Analysis of high-confidence phosphosites shows that phosphorylation on serine residues is most common, followed by threonine and tyrosine phosphorylation. This work forms the basis for a further investigation into the contributions of individual kinases to the overall phosphoproteome of C. difficile and the role of phosphorylation in C. difficile physiology and pathogenesis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Peptoclostridium Difficile (strain 630) (clostridium Difficile)

SUBMITTER: Yassene Mohammed  

LAB HEAD: Paul J. Hensbergen

PROVIDER: PXD029475 | Pride | 2022-02-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
JY Phospho (STY)Sites.txt Txt
L20182002927a.raw Raw
L20182002927b.raw Raw
L20182002929a.raw Raw
L20182002929b.raw Raw
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Publications

Clostridioides difficile Phosphoproteomics Shows an Expansion of Phosphorylated Proteins in Stationary Growth Phase.

Smits Wiep Klaas WK   Mohammed Yassene Y   de Ru Arnoud H AH   Cordo' Valentina V   Friggen Annemieke H AH   van Veelen Peter A PA   Hensbergen Paul J PJ  

mSphere 20220105 1


Phosphorylation is a posttranslational modification that can affect both housekeeping functions and virulence characteristics in bacterial pathogens. In the Gram-positive enteropathogen Clostridioides difficile, the extent and nature of phosphorylation events are poorly characterized, though a protein kinase mutant strain demonstrates pleiotropic phenotypes. Here, we used an immobilized metal affinity chromatography strategy to characterize serine, threonine, and tyrosine phosphorylation in C. d  ...[more]

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