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Differential proteomics of HeLa cells treated with PDS for 24 hours and untreated HeLa cells

ABSTRACT: Pyridostatin (PDS) is a well-known G-quadruplex (G4) inducer and stabilizer, yet its target genes have remained unclear. Herein, combining mass spectrometry based proteomics strategy with bioinformatics analysis, we revealed that PDS significantly downregulated 22 proteins, of which the genes contain rich G4 potential sequences, in HeLa cancer cells, consequently upregulating 16 proteins remarkably. The PDS-regulated proteins appeared to work synergistically to activate cyclin and cell cycle regulation, and to restrain the inhibition of ARE-mediated mRNA decay pathway, suggesting that PDS itself is not a potential anticancer agent, at least towards HeLa cancer.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yinzhu Hou

LAB HEAD: Fuyi Wang

PROVIDER: PXD029521 | Pride | 2022-05-19

REPOSITORIES: Pride

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G-quadruplex inducer/stabilizer pyridostatin targets SUB1 to promote cytotoxicity of a transplatinum complex.

Hou Yinzhu Y Gan Tieliang T Fang Tiantian T Zhao Yao Y Luo Qun Q Liu Xingkai X Qi Luyu L Zhang Yanyan Y Jia Feifei F Han Juanjuan J Li Shumu S Wang Shijun S Wang Fuyi F

Nucleic acids research 20220401 6

Pyridostatin (PDS) is a well-known G-quadruplex (G4) inducer and stabilizer, yet its target genes have remained unclear. Herein, applying MS proteomics strategy, we revealed PDS significantly downregulated 22 proteins but upregulated 16 proteins in HeLa cancer cells, of which the genes both contain a number of G4 potential sequences, implying that PDS regulation on gene expression is far more complicated than inducing/stabilizing G4 structures. The PDS-downregulated proteins consequently upregul ...[more]

PMID: 35258624

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