Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative analysis of global crotonylome of RAW264.7 cells treated by butyrate based on TMT markers

ABSTRACT: The expression of lysozyme was significantly up-regulated in macrophages treated with butyrate. In order to prove the relationship between Lysine Crotonylation modification and lysozyme up-regulation, lysine sites occured croton modification were detected and analyzed.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: Jianmin Wu

LAB HEAD: Jianmin Wu

PROVIDER: PXD029698 | Pride | 2025-06-16

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Butyrate1.zip	Other
	Control1.zip	Other
	combined.zip	Other
	mqpar.xml	Xml

Items per page:

1 - 4 of 4

Similar Datasets

Human-derived PBMCs were stimulated by different groups to kill tumor cells.After 48 hours, PBMCs were taken out, and the enrichment of activated proteins of relevant lymphocytes and the regulation of pathways were examined by proteomics.

Project description:In this study, proteomics was used to explore the activation of lymphocytes and related pathways in PBMCs of different stimulation groups. In our study, proteomics analysis showed that the immune cells in PBMCs activated by stimulation group exhibit improved functions related to white blood cell activation, proliferation and cytotoxicity during biological processes. At the same time, KEGG pathway analysis showed that the signaling pathways’ key proteins for leukocyte transendothelial migration, Th1/Th2 cell differentiation, JAK-STAT (involved in many important biological processes such as cell proliferation, differentiation, apoptosis and immune regulation), DNA replication and Th17 cell differentiation were up-regulated; whereas the key proteins of necroptosis pathways in immune cells were down-regulated.

2023-07-20 | PXD026739 | Pride

CRTAC1 Enhances the Chemosensitivity of Non-Small Cell Lung Cancer to Cisplatin by Eliciting RyR-mediated Calcium Release and Inhibiting Akt1 Expression

Project description:Sensitivity to platinum-based combination chemotherapy is associated with a favorable prognosis in the patients of non-small cell lung cancer (NSCLC). Here, our results obtained from analyses of the Gene Expression Omnibus database of NSCLC patients showed that cartilage acidic protein 1 (CRTAC1) plays a role in the response to platinum-based chemotherapy. Overexpression of CRTAC1 increased sensitivity to cisplatin in vitro, whereas knockdown of CRTAC1 decreased chemosensitivity of NSCLC cells. In vivo mouse experiments showed that CRTAC1 overexpression increased the antitumor effects of cisplatin. CRTAC1 overexpression promoted NFAT transcriptional activation by increasing intracellular Ca2+ levels, thereby inducing its regulated STUB1 mRNA transcription and protein expression, accelerating Akt1 protein degradation, and in turn enhancing cisplatin-induced apoptosis. Taken together, the present results indicate that CRTAC1 overexpression increases the chemosensitivity of NSCLC to cisplatin treatment by inducing Ca2+-dependent Akt1 degradation and apoptosis, suggesting the potential of CRTAC1 as a biomarker for predicting cisplatin chemosensitivity. Our results further reveal that modulating the expression of CRTAC1 could be a new strategy for increasing the efficacy of cisplatin in chemotherapy of NSCLC patients.

2023-08-29 | PXD041782 | Pride

Liquid chromatography-tandem mass spectrometry analysis of bone marrow from the ground or running rats

Project description:Exercise can prevent osteoporosis and improve immune function, but the mechanism remains unclear. To explore the key factors involved in these two processes, we collected bone marrow from ground and running rats and then conducted liquid chromatography-tandem mass spectrometry.

2023-10-17 | PXD033807 | Pride

4D-FastDIA quantitative phosphorylation modification omics analysis before and after expression of Salmonella effector SteC in RAW264.7 cell

Project description:The Salmonella effector SteC is the only protein kinase encoded by Salmonella pathogenicity island 2 that is secreted through the type III secretion system. SteC is known to trigger actin rearrangement via the phosphorylated MEK pathway, and our previous experiments demonstrated that the migration process of macrophages found during Salmonella infection is dependent on the rearrangement of the host cell actin backbone and the action of SteC.To further investigate the target of SteC in the host, we constructed a SteC-RAW264.7 cell line and performed phosphomics analysis using 4D-FastDIA to identify the direct substrates of SteC that trigger macrophage migration and lead to cytoskeletal rearrangement.

2024-02-22 | PXD042038 | Pride

The profound effect of ubiquitin-activating enzyme 1(TgUAE1) on the protein expression of Toxoplasma gondii by 4D label-free quantitative proteomics

Project description:After conditionally knocking down the putative ubiquitin activating enzyme TgUAE1 in Toxoplasma gondii, a series of cutting-edge techniques, including protein extraction, enzymatic digestion, liquid chromatography-mass spectrometry (LC-MS/MS) analysis, and bioinformatics analysis, were synergistically integrated to investigate the quantitative proteome of the samples and identify potential target proteins of TgUAE1

2025-04-28 | PXD045018 | Pride

RNF157 targets RIG-I/DDX58 promotes proliferation in hepatocellular carcinoma

Project description:We first overexpressed RNF157 in Huh-7 cells, followed by collection of the cells and lysis in a lysis buffer (RIPA) supplemented with a protease inhibitor cocktail. Then the cell lysate was centrifuged at 4℃ for 10 minutes to remove cell precipitation. Boil and perform western blot analysis.

2024-10-21 | PXD052805 | Pride

RNA-Seq of FEX1, FEX2 deleted S. cerevisiae (yeast) treated with sodium fluoride against untreated control

Project description:Fluoride is a highly abundant, naturally occurring toxicant that causes oxidative stress, cell cycle arrest, and apoptosis at high concentrations. Our lab has created a fluoride-sensitized yeast model lacking fluoride exporters, FEX1 and FEX2. These yeast undergo cell cycle arrest and apoptosis at over 1000-fold less fluoride concentration than in wild type, and oxidative stress within four hours. To determine the mechanism by which fluoride triggers oxidative stress, we grew cells in 25 mL YPD media at starting O.D. 0.1 in +/- 50 uM NaF. After four hours, the cells were washed twice with sterile water, and RNA was harvested and treated with DNAse. RNA was then sent to the the Yale Center for Genome Analysis for quality analysis with a Bioanalyzer and subsequent poly-A sequencing.

2021-12-02 | E-MTAB-8061 | biostudies-arrayexpress

The global response of Pseudomonas putida to a complex lignolysate

Project description:In this work, we developed processes using ionic liquids to obtain size-defined soluble aromatic-rich streams from sorghum biomass to assess their biocompatibility with P. putida. Growth was unexpectedly higher than that observed on a main aromatic compound. Subsequently, proteomics and metabolomics analysis revealed that BCD liquor contains additional carbon sources except aromatics. Our comparative proteomic analysis first revealed significant up-regulation of a diverse set of proteins in P. putida grown in the complex lignolysate to understand their function and regulation.

2020-04-08 | PXD014285 | Pride

Proteome profile of Traf6-OE OT-I T cells

Project description:Proteome profile of Traf6-OE OT-I T cells and EV OT-I cells after viral transfection

2025-05-06 | PXD039327 | Pride

differentially expressed proteins (DEPs) at the blastocyst stage in the Hspa2-KD and NC groups by applying proteomics technology

Project description:To further investigate the mechanism underlying the ability of HSPA2 to influence cell fate during early embryonic development, we next analyzed differentially expressed proteins (DEPs) at the blastocyst stage in the Hspa2-KD and NC groups by applying proteomics technology.

2025-03-08 | PXD052193 | Pride