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Proteomics analysis haustorium development of Taxillus chinensis

ABSTRACT: Loranthus (Taxillus chinensis) is an important medicinal and parasitic plant that attacks other plants for living. To reveal the mechanisms of haustorium development, we employed an iTRAQ proteomics-based approach to identify differentially abundant proteins (DAPs) of fresh seeds (CK), baby (FB), and adult haustoria (FD).

INSTRUMENT(S):

ORGANISM(S): Taxillus Chinensis

TISSUE(S): Plant Cell, Seed

DISEASE(S): Disease Free

SUBMITTER: limei pan

LAB HEAD: Limei Pan

PROVIDER: PXD029830 | Pride | 2022-10-14

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Combine_Annotation.xls	Xls
	Sangjisheng_T1.raw	Raw
	Sangjisheng_T10.raw	Raw
	Sangjisheng_T11.raw	Raw
	Sangjisheng_T12.raw	Raw

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Publications

Comparative Proteomic Analysis Provides New Insights into the Development of Haustorium in Taxillus chinensis (DC.) Danser.

Pan Limei L Wan Lingyun L Song Lisha L He Lili L Jiang Ni N Long Hairong H Huo Juan J Ji Xiaowen X Hu Fengyun F Fu Jine J Wei Shugen S

BioMed research international 20220728

Taxillus chinensis is an important medicinal and parasitic plant that attacks other plants for living. The development of haustorium is a critical process, imperative for successful parasitic invasion. To reveal the mechanisms underlying haustorium development, we performed an iTRAQ-based proteomics analysis which led to the identification of several differentially abundant proteins (DAPs) in fresh seeds (CK), baby (FB), and adult haustoria (FD). A total of 563 and 785 DAPs were identified and q ...[more]

PMID: 35941969

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Project description:Cuscuta campestris is an obligate stem parasite which uses an organ called the haustoria to divert water and photosynthates from the host. Previously, we have identified that at the haustorial interface between Cuscuta campestris and Arabidopsis thaliana, miRNAs generated by the parasite are able to move into the host and regulate host gene expression. This study identifies how long after attachment does trans-species miRNA transcription begin in Arabidopsis thaliana and Solanum lycopersicum, as well as identifying which stage of haustoria development they become detectable. A time course was performed by harvesting interfaces every 24 hours post attachment, and samples were subjected to RNA extraction and sRNA sequencing. We have identified that in Arabidopsis thaliana, trans-species miRNAs become detectable two days post attachment. In S. lycopersicum, some trans-species miRNAs are detectable one day post attachment, but all become detectable by day 2. Secondary siRNA accumulation was detected four days post attachment in both hosts. In order to determine which stage of haustoria development trans-species miRNAs become detectable, vibratome sectioning was performed on the haustorial interfaces of both hosts. By looking at the morphology of the developing haustoria, it was determined that trans-species miRNAs become detectable during the adhesive phase. This suggests that trans-species miRNA production is one of the first steps of haustoria development, as the parasite tissue has not started to invade host tissue before they become detectable.

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