Dia-PASEF HeLa dilution series 0.2ng - 200ng on Bruker timsTOF Pro 2

ABSTRACT: Different sample preparation methods were tested for HeLa proteome analysis. A sample obtained using sodium deoxycholate-based lysis allowed identification of the highest number of proteins. For this sample, a dilution series was acquired in triplicates ranging from 0.2ng to 200ng. All measurements were performed on Bruker timsTOF Pro 2 operated in dia-PASEF mode and analysed library-free using DIA-NN 1.8.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Vadim Demichev  

LAB HEAD: Markus Ralser

PROVIDER: PXD029836 | Pride | 2022-06-20

REPOSITORIES: Pride