Proteomics

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Partial characterization of the glycoconjugates of Trypanosoma theileri


ABSTRACT: Here, we have started to characterise the surface molecules of T. theileri at the biochemical level. We have applied the same differential solvent extraction and hydrophobic interaction chromatography protocols, developed by Almeida and colleagues [13,14] to those used to isolate the GPI-mucin and GIPL fractions of T. theileri. As a positive control, we processed epimastigotes of T. cruzi Silvio X10/7 strain alongside tissue culture forms of T. theileri Edinburgh strain and so also report on the surface molecules of the Silvio X10/7 strain of T. cruzi for the first time.

INSTRUMENT(S):

ORGANISM(S): Trypanosoma Brucei

SUBMITTER: Michele Tinti  

LAB HEAD: Professor Sir Mike Ferguson

PROVIDER: PXD029837 | Pride | 2025-09-29

REPOSITORIES: Pride

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Publications

Characterization of the major surface glycoconjugates of Trypanosoma theileri.

Nagar Rupa R   Hambleton Isobel I   Tinti Michele M   Carrington Mark M   Ferguson Michael A J MAJ  

Molecular and biochemical parasitology 20230829


Trypanosoma theileri maintains a long-term extracellular infection with a low parasitaemia in bovids. The surface of this parasite is predicted to be decorated with several surface molecules including membrane surface proteases (MSPs), trans-sialidases and T. theileri putative surface proteins (TTPSPs). However, there are no experimental data to verify this hypothesis. Here, we have purified and partially characterized the surface glycoconjugates of T. theileri using biochemical and mass spectro  ...[more]

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