Dataset Information

Proteome dataset of cultured human bone marrow derived stromal (stem) cells, adipocytes and osteoblasts

ABSTRACT: Mesenchymal stromal stem cells (MSCs) gain increasing focus in the field of regenerative medicine due to their differentiational abilities into chondrocytes, adipocytes and osteoblastic cells. However, it is apparent that the transformation processes are extremely complex, cause cellular heterogeneity and need further insights into functional annotation. The aim of the study was to characterize differences between MSCs and cells after adipogenic or osteoblastic differentiation at the proteome level. For this, we analysed the proteome of isolated and cultured MSCs and after differentiation into adipogenic and osteoblastic cell lineages using electrospray ionization tandem mass spectrometry in data-independent acquisition mode. Protein inference and quantification was performed using deep neural networks in library free mode. By jointly using strict differential expression analysis and machine learning algorithms, two cell type specific marker protein panels for the osteoblastic and the adipocytic lineage were identified which allow for distinguishment of the three cell types.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Thorben Sauer

LAB HEAD: Timo Gemoll

PROVIDER: PXD029900 | Pride | 2022-03-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20191021_BonebankJustina_AD_11_SWATH.wiff	Wiff
	20191021_BonebankJustina_AD_11_SWATH.wiff.scan	Wiff
	20191021_BonebankJustina_AD_13_SWATH.wiff	Wiff
	20191021_BonebankJustina_AD_13_SWATH.wiff.scan	Wiff
	20191021_BonebankJustina_AD_15_SWATH.wiff	Wiff

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Publications

Protein Expression of AEBP1, MCM4, and FABP4 Differentiate Osteogenic, Adipogenic, and Mesenchymal Stromal Stem Cells.

Sauer Thorben T Facchinetti Giulia G Kohl Michael M Kowal Justyna M JM Rozanova Svitlana S Horn Julia J Schmal Hagen H Kwee Ivo I Schulz Arndt-Peter AP Hartwig Sonja S Kassem Moustapha M Habermann Jens K JK Gemoll Timo T

International journal of molecular sciences 20220225 5

Mesenchymal stem cells (MSCs) gain an increasing focus in the field of regenerative medicine due to their differentiation abilities into chondrocytes, adipocytes, and osteoblastic cells. However, it is apparent that the transformation processes are extremely complex and cause cellular heterogeneity. The study aimed to characterize differences between MSCs and cells after adipogenic (AD) or osteoblastic (OB) differentiation at the proteome level. Comparative proteomic profiling was performed usin ...[more]

PMID: 35269711

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