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Mass spectrometry-based characterization of SET1/COMPASS complex in Caenorhabditis elegans

ABSTRACT: Changes in histone post-translational modifications are associated with aging through poorly defined mechanisms. Histone 3 lysine 4 (H3K4) methylation at promoters is deposited by SET1 family methyltransferases acting within conserved multiprotein complexes known as COMPASS. Using co-immunopurification coupled to mass spectrometry-based proteomics, we characterized complex members and binding partners in various genetic contexts, using WDR-5 or CFP-1 proteins as baits.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive HF

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Embryo

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD029904 | Pride | 2021-12-08

REPOSITORIES: Pride

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