Proteomics

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Lysosomal damage proteomics in HeLa cells


ABSTRACT: To identify ubiquitylation targets following lysosomal damage in HeLa cells treated with LLOMe we performed quantitative ubiquitin-remmnant (diGly) profilig coupled to mass spectrometry (MS). In addition, we performed APEX2-based proximity biotinylation followed by MS analysis to identify proximity partners of one of the ubiquitylation targets (CNN2).

INSTRUMENT(S): Q Exactive HF, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Christian Behrends  

LAB HEAD: Christian Behrends

PROVIDER: PXD029926 | Pride | 2022-06-23

REPOSITORIES: Pride

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