Dataset Information

Detection SARS-CoV-2 infection in mice by ATR-FTIR

ABSTRACT: Vero-E6 cell supernatant, mice lavage and human saliva were evaulated after exposure to SARS-CoV-2 via ATR-FTIR spectoscopy. To provide further information about the physiological response, proteins contained in the mouse lavage were assessed via shotgun proteomics (data shown here). Mice were exposed to active SARS-CoV-2 or UV-inactivated SARS-CoV-2 for up to 4 days, lavage collected at day 0, day 2 and day 4 (D4). Lavage proteins were pooled for day 0 (due to low protein abundance) and compared to UV-I-SARS-CoV-2(n=4)and SARS-CoV-2(n=8)samples.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Covid-19

SUBMITTER: Harley Robinson

LAB HEAD: Michelle M. Hill

PROVIDER: PXD030012 | Pride | 2021-12-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Oxidation__M_Sites.txt	Txt
	Plus_20211021_QIMR_HR_AlzD_Cov_CoVSal_D4-10_Sample.raw	Raw
	Plus_20211021_QIMR_HR_AlzD_Cov_CoVSal_D4-11_Sample.raw	Raw
	Plus_20211021_QIMR_HR_AlzD_Cov_CoVSal_D4-12_Sample.raw	Raw
	Plus_20211021_QIMR_HR_AlzD_Cov_CoVSal_D4-13_Sample.raw	Raw

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Publications

Pathophysiological Response to SARS-CoV-2 Infection Detected by Infrared Spectroscopy Enables Rapid and Robust Saliva Screening for COVID-19.

Kazmer Seth T ST Hartel Gunter G Robinson Harley H Richards Renee S RS Yan Kexin K van Hal Sebastiaan J SJ Chan Raymond R Hind Andrew A Bradley David D Zieschang Fabian F Rawle Daniel J DJ Le Thuy T TT Reid David W DW Suhrbier Andreas A Hill Michelle M MM

Biomedicines 20220201 2

Fourier transform infrared (FTIR) spectroscopy provides a (bio)chemical snapshot of the sample, and was recently used in proof-of-concept cohort studies for COVID-19 saliva screening. However, the biological basis of the proposed technology has not been established. To investigate underlying pathophysiology, we conducted controlled infection experiments on Vero E6 cells in vitro and K18-hACE2 mice in vivo. Potentially infectious culture supernatant or mouse oral lavage samples were treated with ...[more]

PMID: 35203562

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