Detection SARS-CoV-2 infection in mice by ATR-FTIR

ABSTRACT: Vero-E6 cell supernatant, mice lavage and human saliva were evaulated after exposure to SARS-CoV-2 via ATR-FTIR spectoscopy. To provide further information about the physiological response, proteins contained in the mouse lavage were assessed via shotgun proteomics (data shown here). Mice were exposed to active SARS-CoV-2 or UV-inactivated SARS-CoV-2 for up to 4 days, lavage collected at day 0, day 2 and day 4 (D4). Lavage proteins were pooled for day 0 (due to low protein abundance) and compared to UV-I-SARS-CoV-2(n=4)and SARS-CoV-2(n=8)samples.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Covid-19

SUBMITTER: Harley Robinson  

LAB HEAD: Michelle M. Hill

PROVIDER: PXD030012 | Pride | 2021-12-17

REPOSITORIES: Pride