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Detection SARS-CoV-2 infection in mice by ATR-FTIR

ABSTRACT: Vero-E6 cell supernatant, mice lavage and human saliva were evaulated after exposure to SARS-CoV-2 via ATR-FTIR spectoscopy. To provide further information about the physiological response, proteins contained in the mouse lavage were assessed via shotgun proteomics (data shown here). Mice were exposed to active SARS-CoV-2 or UV-inactivated SARS-CoV-2 for up to 4 days, lavage collected at day 0, day 2 and day 4 (D4). Lavage proteins were pooled for day 0 (due to low protein abundance) and compared to UV-I-SARS-CoV-2(n=4)and SARS-CoV-2(n=8)samples.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Covid-19

SUBMITTER: Harley Robinson

LAB HEAD: Michelle M. Hill

PROVIDER: PXD030012 | Pride | 2021-12-17

REPOSITORIES: Pride

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Project description:Background: Type I interferons (IFNs) are essential to the clearance of viral diseases, in part by initiating upregulation of IFN regulated genes (IRGs). A clear distinction between genes upregulated directly by virus and genes upregulated by secondary IFN production has not been made. Here we investigated the genes regulated by IFN-a2b compared to the genes regulated by SARS-CoV infection in ferrets. Methods: We characterized early host immune responses in peripheral blood and lung necropsies of ferrets injected with IFN-a2b or infected with SARS-CoV/Tor 2 strain, using microarray analysis on the Affymetrix platform. Results: We identified a common IRG signature that was upregulated in both SARS-CoV infected ferrets as well as in ferrets injected with IFN-a2b. We also identified unique patterns of gene expression for leukocyte activation, cell adhesion and complement pathways between IFN-a2b injection and SARS-CoV infection. Conclusions: Our results define the effects of IFN-a2b on the immune system of ferrets highlighting genes regulated by IFN during SARS-CoV infection. We have shown the similarities and differences of top funcional gene groups as well as pathways that play key roles in early immune responses in ferrets in response to IFN-a2b or SARS-CoV. Key words: ferret, gene expression, SARS, interferon. Keywords: time course In experiments with IFN-a2b, for peripheral blood, 15 ferrets were randomly allocated to 3 groups: Day 0, 5 ferrets (no IFN injection), day 1, 6 ferrets (injected), and day 2, 4 ferrets (injected). For lung necropsies of injected ferrets with IFN-a2b, we used 12 ferrets in 3 groups: 4 ferrets, day 0 (no IFN injection), 4 ferrets, day 1 (injected) and 4 ferrets, day 2 (injected). Experimental groups for SARS-CoV infection was as follows: For peripheral blood, 3 and 4 ferrets for day 0 (no infection) and day 2 (infection) respectively. For lung neceropsies, a total of 9 ferrets in 3 groups, each with 3 replicates for day 0 (no infection), day 1 (infection) and day 2 (infection).

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Detection SARS-CoV-2 infection in mice by ATR-FTIR

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