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Parallel Reaction Monitoring of 42 peptide pairs with 15N metabolic labeling

ABSTRACT: To examine the properties of 14N and 15N labeled peptides from 15 metabolic labeling samples, we set up targeted quantification using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer. We first examined the co-elution of light and heavy peptide forms. Furthermore, we examined the fragment spectra of the light and heavy peptides and compared the pattern of the fragments.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Shouling Xu

LAB HEAD: Shouling Xu

PROVIDER: PXD030112 | Pride | 2022-06-09

REPOSITORIES: Pride

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Application of Parallel Reaction Monitoring in 15N Labeled Samples for Quantification.

Reyes Andres V AV Shrestha Ruben R Baker Peter R PR Chalkley Robert J RJ Xu Shou-Ling SL

Frontiers in plant science 20220503

Accurate relative quantification is critical in proteomic studies. The incorporation of stable isotope 15N to plant-expressed proteins in vivo is a powerful tool for accurate quantification with a major advantage of reducing preparative and analytical variabilities. However, 15N labeling quantification has several challenges. Less identifications are often observed in the heavy-labeled samples because of incomplete labeling, resulting in missing values in reciprocal ...[more]

PMID: 35592564

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