Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: Gereon Poschmann
LAB HEAD: Gereon Poschmann
PROVIDER: PXD030139 | Pride | 2022-10-13
REPOSITORIES: Pride
Müller Lisa L Ptok Johannes J Nisar Azlan A Antemann Jennifer J Grothmann Ramona R Hillebrand Frank F Brillen Anna-Lena AL Ritchie Anastasia A Theiss Stephan S Schaal Heiner H
Nucleic acids research 20220801 15
Correct pre-mRNA processing in higher eukaryotes vastly depends on splice site recognition. Beyond conserved 5'ss and 3'ss motifs, splicing regulatory elements (SREs) play a pivotal role in this recognition process. Here, we present in silico designed sequences with arbitrary a priori prescribed splicing regulatory HEXplorer properties that can be concatenated to arbitrary length without changing their regulatory properties. We experimentally validated in silico predictions in a massively parall ...[more]