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Co-IP of v-ATPase subcomplexes from MEFs under amino acid starvation and restimulation

ABSTRACT: v-ATPase Vo and V1 domains were isolated via Co-IP from MEFs expressing HA-Atp6V1B2 and FLAG-Atp6VoaA3 using HA- and FLAG-tag antibodies. Treatment groups were full medium (control), amino acid starvation (60 min) and restimulation (30 min). Cells were grown in SILAC medium for 3 passages.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Fibroblast, Cell Culture

SUBMITTER: Wilhelm Palm

LAB HEAD: Wilhelm Palm

PROVIDER: PXD030174 | Pride | 2022-08-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ER0422-14.xml	Xml
	ER0422_new.fasta	Fasta
	ER0422_txt.zip	Other
	Sample2rawfile_ER0422.csv	Csv
	UP000000589_10090.fasta	Fasta

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