Full length protein sequencing method based on less specific and unspecific hydrolysis strategies 2
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ABSTRACT: We describe a new strategy for LC-MS/MS based full length protein sequencing. Protein samples were unspecific hydrolyzed by three different methods(proteinase K, papain and microwave-assisted acid hydrolysis) to improve overlapping degree of peptides. After LC-MS/MS analysis, peptide sequences were gernated by de novo peptide sequencing program Pnovo. A new sequence assembly program, based on de brujin graph and Overlap-Layout-Consensus strategy, was desined to generate full length protein sequence using Pnovo results.
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Equus Caballus (horse) Homo Sapiens (human) Bos Taurus (bovine)
TISSUE(S): Cell Culture
SUBMITTER: zhou zhong hua
LAB HEAD: Gong Zhang
PROVIDER: PXD030203 | Pride | 2022-02-15
REPOSITORIES: Pride
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