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Multi-omic characterisation of Streptomyces hygroscopicus NRRL 30439: detailed assessment of its secondary metabolic potential

ABSTRACT: Time course intracellular proteomics of Streptomyces hygroscopicus NRRL 30439 (S. inhibens NRRL30439, CP084681) during submerged culture fermentation. Label free quantification of LC-MSMS spectra was used for protein expression profiling to support primary and secondary metabolic functionalities.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Streptomyces Inhibens

SUBMITTER: Craig Barry

LAB HEAD: Esteban Marcellin

PROVIDER: PXD030257 | Pride | 2025-06-03

REPOSITORIES: Pride

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			Action	DRS
	20180914_T1_005.raw	Raw
	20180914_T1_006.raw	Raw
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	20180914_T1_009.raw	Raw

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Multi-omic characterisation of <i>Streptomyces hygroscopicus</i> NRRL 30439: detailed assessment of its secondary metabolic potential.

Barry Craig P CP Gillane Rosemary R Talbo Gert H GH Plan Manual M Palfreyman Robin R Haber-Stuk Andrea K AK Power John J Nielsen Lars K LK Marcellin Esteban E

Molecular omics 20220328 3

The emergence of multidrug-resistant pathogenic bacteria creates a demand for novel antibiotics with distinct mechanisms of action. Advances in next-generation genome sequencing promised a paradigm shift in the quest to find new bioactive secondary metabolites. Genome mining has proven successful for predicting putative biosynthetic elements in secondary metabolite superproducers such as <i>Streptomycetes</i>. However, genome mining approaches do not inform whether biosynthetic gene clusters are ...[more]

PMID: 34989730

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