Dataset Information

Synechococcus elongatus PCC 7942 proteomics

ABSTRACT: The first comparative proteomics is to understand protein profile differences between high limonene production phase and low limonene production phase in limonene producing strain L1118. The second comparative proteomics is to understand protein profile differences between L1118 and sucrose mutant Lsps.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Synechococcus Elongatus (strain Pcc 7942) (anacystis Nidulans R2)

TISSUE(S): Photosynthetic Cell, Cell Culture

SUBMITTER: Bin Long

LAB HEAD: Joshua S. Yuan

PROVIDER: PXD030282 | Pride | 2023-10-24

REPOSITORIES: Pride

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Publications

Altered Carbon Partitioning Enhances CO<sub>2</sub> to Terpene Conversion in Cyanobacteria.

Li Man M Long Bin B Dai Susie Y SY Golden James W JW Wang Xin X Yuan Joshua S JS

Biodesign research 20220207

Photosynthetic terpene production represents one of the most carbon and energy-efficient routes for converting CO<sub>2</sub> into hydrocarbon. In photosynthetic organisms, metabolic engineering has led to limited success in enhancing terpene productivity, partially due to the low carbon partitioning. In this study, we employed systems biology analysis to reveal the strong competition for carbon substrates between primary metabolism (e.g., sucrose, glycogen, and protein synthesis) and terpene bi ...[more]

PMID: 37850123

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Project description:Biological CO2 mitigation by photosynthetic microorganisms has emerged as a promising approach for generating biomass-based energy during the course of CO2 fixation. Additionally, cyanobacteria-based biofuels have also garnered immense attention lately because of the depleting fossil fuel reserves and the growing energy demands. Synechococcus elongatus PCC 11801, a fast-growing, high CO2 tolerant, novel isolate of cyanobacteria, is an interesting candidate for metabolic engineering applications. Since under laboratory conditions, it exhibited a high level of tolerance to different environmental stresses (CO2, light, temperature, salts and butanol), it seemed like an encouraging prospect for the production of fuels and other industrially relevant chemicals. This is the first-ever functional global proteomics investigation of Synechococcus elongatus PCC 11801 isolate grown at elevated CO2 levels using high-throughput iTRAQ approach. Three independent biological replicates were analyzed and a total of 861 proteins were identified, out of which 492 proteins were found to be present in all the three replicates. Of these, 248 proteins showing the same trend across the three replicates (≥1.5 fold up-regulation or ≤0.66 fold down-regulation) were chosen for pathway analysis. The metabolic responses were marked with a down-regulation of inorganic carbon transporters alongside an induction of nitrogen transport and absorption proteins in order to uphold the apposite carbon-nitrogen equilibrium. Further acclimation progression exposed an increased expression of proteins taking part in photosynthesis and generation of light harvesting pigments such as chlorophyll. Similarly, a downshift of proteins involved in photoprotection and defense against ROS (reactive oxygen species) was observed. Another principal discovery was the perturbation in expression of proteins belonging to central metabolic pathways like glycolysis, TCA cycle, pentose phosphate pathway as a coping mechanism to high CO2 stress. Further, validation studies were carried out using MRM assays and western blotting of key altered proteins.

Dataset Information

Synechococcus elongatus PCC 7942 proteomics

Publications

Altered Carbon Partitioning Enhances CO<sub>2</sub> to Terpene Conversion in Cyanobacteria.

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