Fasciola hepatica cathepsin L zymogens: immuno-proteomic evidence for highly immunogenic zymogen-specific conformational epitopes to support diagnostics development

ABSTRACT: We conducted immuno-proteomic investigations of recombinant and native procathepsins ((r)FhpCL1) and counterpart polyclonal antibodies to a recombinant mutant procathepsin L (anti-rFhΔpCL1). Proteomics including gel electrophoresis and liquid chromatography tandem mass spectrometry were used for formal identification and in the determination of sample compositions. We have confirmed recombinant and native cathepsin L zymogens contain conserved, highly antigenic epitopes that are conformationally dependent.

INSTRUMENT(S):

ORGANISM(S): Fasciola Hepatica (liver Fluke)

DISEASE(S): Fascioliasis

SUBMITTER: Clare Collett  

LAB HEAD: Professor Peter Brophy

PROVIDER: PXD030293 | Pride | 2022-07-19

REPOSITORIES: Pride