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Enhanced competition at the nano-bio interface enables comprehensive characterization of protein corona dynamics and deep coverage of proteomes

ABSTRACT: We demonstrate the importance of the protein to NP-surface ratio (P/NP) and model protein corona formation dynamics, which determine the competition between proteins for binding.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Theo Platt

LAB HEAD: Daniel Hornburg

PROVIDER: PXD030327 | Pride | 2022-09-02

REPOSITORIES: Pride

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Enhanced Competition at the Nano-Bio Interface Enables Comprehensive Characterization of Protein Corona Dynamics and Deep Coverage of Proteomes.

Ferdosi Shadi S Stukalov Alexey A Hasan Moaraj M Tangeysh Behzad B Brown Tristan R TR Wang Tianyu T Elgierari Eltaher M EM Zhao Xiaoyan X Huang Yingxiang Y Alavi Amir A Lee-McMullen Brittany B Chu Jessica J Figa Mike M Tao Wei W Wang Jian J Goldberg Martin M O'Brien Evan S ES Xia Hongwei H Stolarczyk Craig C Weissleder Ralph R Farias Vivek V Batzoglou Serafim S Siddiqui Asim A Farokhzad Omid C OC Hornburg Daniel D

Advanced materials (Deerfield Beach, Fla.) 20220920 44

Introducing engineered nanoparticles (NPs) into a biofluid such as blood plasma leads to the formation of a selective and reproducible protein corona at the particle-protein interface, driven by the relationship between protein-NP affinity and protein abundance. This enables scalable systems that leverage protein-nano interactions to overcome current limitations of deep plasma proteomics in large cohorts. Here the importance of the protein to NP-surface ratio (P/NP) is demonstrated and protein c ...[more]

PMID: 35986672

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