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Identification of TBBPA-degraders in anaerobic bioreactor at environmentally relevant concentrations by combined protein-based stable isotope probing and metagenomics

ABSTRACT: The aim of this study was to identify TBBPA-degrading organisms in a complex microbial community by a metagenome-based functional metaproteomic approach, using protein-based stable isotope probing (protein-SIP). Firstly, the degradation kinetics were evaluated in order to simulate the decrease of residual mass of the labelled compound based on experimental data. In sequence, a metagenome was generated, and biomass was collected in different time-points for protein-SIP in incubations with 13C-TBBPA. This approach allowed for the identification organisms assimilating labelled carbon from the cometabolic degradation of a micropollutant.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Anaerobic Digester Metagenome

SUBMITTER: Jan Struckmann Poulsen

LAB HEAD: Jeppe Lund Nielsen

PROVIDER: PXD030335 | Pride | 2022-09-12

REPOSITORIES: Pride

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