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MiniTurbo-based interactomics of two plasma membrane-localized SNARE proteins in Marchantia polymorpha

ABSTRACT: • Marchantia polymorpha is a model liverwort and its overall low genetic redundancy is advantageous for dissecting complex pathways. Proximity-dependent in vivo biotin-labelling methods have emerged as powerful interactomics tools in recent years. However, interactomics studies applying proximity labelling are currently limited to angiosperm species in plants. • Here, we established and evaluated a miniTurbo-based interactomics method in M. polymorpha using MpSYP12A and MpSYP13B, two plasma membrane-localized SNARE proteins, as baits. • We show that our method yields a manifold of potential interactors of MpSYP12A and MpSYP13B compared to a co-immunoprecipitation approach. Our method could capture specific candidates for each SNARE. • We conclude that a miniTurbo-based method is a feasible tool for interactomics in M. polymorpha, potentially applicable to other model bryophytes. Our interactome dataset on MpSYP12A and MpSYP13B will be a useful resource to elucidate the evolution of SNARE functions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Marchantia Polymorpha

TISSUE(S): Plant Cell

SUBMITTER: Sara Christina Stolze

LAB HEAD: Hirofumi Nakagami

PROVIDER: PXD030429 | Pride | 2022-04-01

REPOSITORIES: Pride

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