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Identification of Setdb1 interaction proteins in neural stem cells

ABSTRACT: Setdb1 is an epigenetic factors catalyzing modification of H3K9me3. Expression of its gene is localized to embryonic neural cells during vertebrate embryogenesis, suggesting its role in regulating neural stemness. The project is to identify the interaction partners of Setdb1, by which Setdb1 regulates neural stemness in neural stem cells.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Ying Cao

LAB HEAD: Ying Cao

PROVIDER: PXD030453 | Pride | 2022-08-12

REPOSITORIES: Pride

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Neural stemness unifies cell tumorigenicity and pluripotent differentiation potential.

Zhang Min M Liu Yang Y Shi Lihua L Fang Lei L Xu Liyang L Cao Ying Y

The Journal of biological chemistry 20220604 7

Neural stemness is suggested to be the ground state of tumorigenicity and pluripotent differentiation potential. However, the relationship between these cell properties is unclear. Here, by disrupting the neural regulatory network in neural stem and cancer cells and by serial transplantation of cancer cells, we show that tumorigenicity and pluripotent differentiation potential are coupled cell properties unified by neural stemness. We show that loss of neural stemness via inhibition of SETDB1, a ...[more]

PMID: 35671824

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