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Impact of nitrate on the proteome of the primary root tip of Medicago truncatula

ABSTRACT: The impact of nitrate on the proteome of the primary root tip of Medicago truncatula were compared in R108, a wild and sensitive genotype, and npf6.8, a mutant and insensitive line by shotgun analysis. Each genotype were cultivated in N-free condition or with 5mM of nitrate in three independent biological replicate. Sample collection was performed of 10 days old seedlings

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Medicago Truncatula (barrel Medic) (medicago Tribuloides)

TISSUE(S): Root

SUBMITTER: Thierry Balliau

LAB HEAD: Dr Françoise Montirchard

PROVIDER: PXD030547 | Pride | 2022-05-20

REPOSITORIES: Pride

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The Nitrate Transporter MtNPF6.8 Is a Master Sensor of Nitrate Signal in the Primary Root Tip of <i>Medicago truncatula</i>.

Zang Lili L Tarkowski Łukasz Paweł ŁP Morère-Le Paven Marie-Christine MC Zivy Michel M Balliau Thierry T Clochard Thibault T Bahut Muriel M Balzergue Sandrine S Pelletier Sandra S Landès Claudine C Limami Anis M AM Montrichard Françoise F

Frontiers in plant science 20220318

Nitrate is not only an essential nutrient for plants, but also a signal involved in plant development. We have previously shown in the model legume <i>Medicago truncatula</i>, that the nitrate signal, which restricts primary root growth, is mediated by MtNPF6.8, a nitrate transporter. Nitrate signal also induces changes in reactive oxygen species accumulation in the root tip due to changes in cell wall peroxidase (PODs) activity. Thus, it was interesting to determine the importance of the role o ...[more]

PMID: 35371178

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