Project description:Reanalysis of a synthetic crosslinked peptide library datasets with DSS (non-cleavable), DSSO and DSBU (MS-cleavable) cross linkers from Beveridge et al., Nat. Commun., 2020 (PXD014337)
Project description:Reanalysis of a synthetic crosslinked peptide library dataset. The three replicates of the XL-MS experiment with the non-cleavable cross-linker DSS from the dataset published in Beveridge et al., Nature Commun., 2020 (PRIDE project PXD014337) were searched with the XL-MS identification tool OpenPepXL for benchmarking purposes.
Project description:The CTC-iChip microfluidic device [PMID: 23552373 ] enables isolation of rare viable circulating tumor cells (CTCs) directly from whole blood specimens of patients with cancer. Reanalysis of freshly isolated CTC from 31 women with hormone receptor positive metastatic breast cancer.
Project description:Ants are among the most successful animals on earth, with societies of a complexity that rivals our own. These societies are characterized by reproductive division of labor between female queens that can live several years and lay thousands of eggs per day, workers that live only a few months and are sterile, and males that live only a few weeks and do not participate in colony tasks. These striking differences in lifespan and roles are echoed by extensive morphological and physiological divergence. Using the fire ant, Solenopsis invicta, we conduct the first genome-wide survey of developmental gene expression levels over 20 time-points from larval to adult stages in workers, queens and males Three castes: Worker, queen & male; four colonies used for each caste (12 colonies total). Timepoints: 0h, 3h, 6h, 12h, 18h, 24h, 36h and 48h (hour timepoints) and once every 24hours until eclosion (day timepoints). Eclosion occured after 15 days for male (21 timepoints), 14 days for queen (20 timepoints) and 12 days for worker larvae (19 timepoints). We used a loop design with direct comparisons of consecutive samples within each cast: two loops in one Dye-direction, two loops in the other for balance. Additionally, 3-5 hybridizations against an unrelated Reference RNA were performed within each replicate loop. Microarray batch is indicated in the description column