Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Eclipse
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture
SUBMITTER: Thermo Fisher Scientific Center for Multiplexed Proteomics
LAB HEAD: William Hahn
PROVIDER: PXD030599 | Pride | 2022-09-06
REPOSITORIES: Pride
So Jonathan J Mabe Nathaniel W NW Englinger Bernhard B Chow Kin-Hoe KH Moyer Sydney M SM Yerrum Smitha S Trissal Maria C MC Marques Joana G JG Kwon Jason J JJ Shim Brian B Pal Sangita S Panditharatna Eshini E Quinn Thomas T Schaefer Daniel A DA Jeong Daeun D Mayhew David L DL Hwang Justin J Beroukhim Rameen R Ligon Keith L KL Stegmaier Kimberly K Filbin Mariella G MG Hahn William C WC
JCI insight 20221010 19
Collateral lethality occurs when loss of a gene/protein renders cancer cells dependent on its remaining paralog. Combining genome-scale CRISPR/Cas9 loss-of-function screens with RNA sequencing in over 900 cancer cell lines, we found that cancers of nervous system lineage, including adult and pediatric gliomas and neuroblastomas, required the nuclear kinase vaccinia-related kinase 1 (VRK1) for their survival in vivo. VRK1 dependency was inversely correlated with expression of its paralog VRK2. VR ...[more]