Name: High-throughput identification of RNA localization elements reveals a regulatory role for A/G rich sequences
Keywords: Lc-ms/ms

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High-throughput identification of RNA localization elements reveals a regulatory role for A/G rich sequences

ABSTRACT: This experiment aims to identify the RNA-binding proteome that is pulled-down from a cellular lysate with biotinylated RNA probes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Andreas Moor

LAB HEAD: Andreas Moor

PROVIDER: PXD030720 | Pride | 2022-09-21

REPOSITORIES: Pride

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High-throughput identification of RNA localization elements in neuronal cells.

Arora Ankita A Castro-Gutierrez Roberto R Moffatt Charlie C Eletto Davide D Becker Raquel R Brown Maya M Moor Andreas E AE Russ Holger A HA Taliaferro J Matthew JM

Nucleic acids research 20221001 18

Hundreds of RNAs are enriched in the projections of neuronal cells. For the vast majority of them, though, the sequence elements that regulate their localization are unknown. To identify RNA elements capable of directing transcripts to neurites, we deployed a massively parallel reporter assay that tested the localization regulatory ability of thousands of sequence fragments drawn from endogenous mouse 3' UTRs. We identified peaks of regulatory activity within several 3' UTRs and found that seque ...[more]

PMID: 36107770

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