Dataset Information

The expression of BDNF and the levels of this neurotrophin influence the behavioural response in Zebrafish

ABSTRACT: Experimental evidence suggests that environmental stress conditions can alter the expression of BDNF and the levels of this neurotrophin influence the behavioural response in mammalian models. Recently it has been demonstrated that the exposure to 34° C for 21 days alters the brain proteome and behaviour in zebrafish. The aim of this work was to investigate the role of BDNF in the nervous system of adult zebrafish under control and heat treatment conditions. For this purpose, wild type (WT), heterozygous BDNF+/- (HT) and knock out BDNF-/- (KO) zebrafish were kept for 21 days at 26° C or 34° C and then euthanized for brain analysis or subjected to behavioural tests (Y-maze test, novel tank test, light and dark test, social preference test, mirror biting test) used for assessing behavioural aspects such as boldness, anxiety, social preference, aggressivity, interest for the novel environment and exploration. RTqPCR analysis showed the reduction of gene expression of BDNF and its receptors after heat treatment. Moreover, proteomic analysis and behavioural tests showed genotype- and temperature-dependent effects on brain proteome and behaviour of zebrafish.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Brain

SUBMITTER: Francesca Grassi Scalvini

LAB HEAD: Francesca Grassi Scalvini

PROVIDER: PXD030733 | Pride | 2022-06-09

REPOSITORIES: Pride

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Publications

Brain Proteome and Behavioural Analysis in Wild Type, BDNF+/- and BDNF-/- Adult Zebrafish (Danio rerio) Exposed to Two Different Temperatures.

Maffioli Elisa E Angiulli Elisa E Nonnis Simona S Grassi Scalvini Francesca F Negri Armando A Tedeschi Gabriella G Arisi Ivan I Frabetti Flavia F D'Aniello Salvatore S Alleva Enrico E Cioni Carla C Toni Mattia M

International journal of molecular sciences 20220517 10

Experimental evidence suggests that environmental stress conditions can alter the expression of BDNF and that the expression of this neurotrophin influences behavioural responses in mammalian models. It has been recently demonstrated that exposure to 34 °C for 21 days alters the brain proteome and behaviour in zebrafish. The aim of this work was to investigate the role of BDNF in the nervous system of adult zebrafish under control and heat treatment conditions. For this purpose, zebrafish from t ...[more]

PMID: 35628418

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Project description:Social plasticity is a pervasive feature of animal behavior. Animals adjust the expression of their social behavior to the daily changes in social life and to transitions between life-history stages, and the ability to change in these ways impacts their Darwinian fitness. This behavioral plasticity may be achieved either by rewiring or by biochemically switching nodes of the neural network underlying the social behavior in response to perceived social information. Independent of the proximate mechanisms, at the neuromolecular level social plasticity relies on the regulation of gene expression, such that different neurogenomic states emerge in response to different social stimuli and the switches between states are orchestrated by signaling pathways that interface the social environment and the genotype. Here, we test this hypothesis by characterizing the changes in the brain profile of gene expression in response to social odors in the Mozambique Tilapia, Oreochromis mossambicus. This species has a rich repertoire of social behaviors during which both visual and chemical information are conveyed to conspecifics. Specifically, dominant males increase their urination frequency during agonist encounters and during courtship to convey chemical information reflecting their dominance status. We recorded electro-olfactograms to test the extent to which the olfactory epithelium can discriminate between olfactory information from dominant and subordinate males as well as from pre- and post-spawning females. We then performed a genome-scale gene expression analysis of the olfactory bulb and the olfactory cortex homolog in order to identify the neuromolecular systems involved in processing these social stimuli. Our results show that different olfactory stimuli from conspecificsM-bM-^@M-^Y have a major impact in the brain transcriptome, with different chemical social cues eliciting specific patterns of gene expression in the brain. These results confirm the role of rapid changes in gene expression in the brain as a genomic mechanism underlying behavioural plasticity and reinforce the idea of an extensive transcriptional plasticity of cichlid genomes, especially in response to rapid changes in their social environment. Brain samples from 40 African cichlid males, Oreochromis mossambicus were collected after stimulation with different social olfactory stimuli. Samples were collected from 2 brain areas: BO and Dp after males were exposed to dominant (DOM) and subordinate (SUB) male urine and pre- (PRE) and post-ovulatory (POST) female scent. In OB 5 replicates were collected from males exposed to DOM and 6 to the other stimuli. For Dp 5 replicates were collected from males exposed to DOM and POST, 4 to SUB and 6 to PRE.

Dataset Information

The expression of BDNF and the levels of this neurotrophin influence the behavioural response in Zebrafish

Publications

Brain Proteome and Behavioural Analysis in Wild Type, BDNF<sup>+/-</sup> and BDNF<sup>-/-</sup> Adult Zebrafish (<i>Danio rerio</i>) Exposed to Two Different Temperatures.

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