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Ubiquitomics and proteomics analysis of porcine articular cartilage post mechanical injury

ABSTRACT: We describe ubiquitomics and proteomics analysis of porcine articular cartilage post mechanical injury. Analysis showed ubiquitin peptides differentially enriched post injury compared to non injured controls.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Articular Cartilage, Articular Chondrocyte

SUBMITTER: Heba Ismail

LAB HEAD: Heba Ismail

PROVIDER: PXD030817 | Pride | 2022-12-19

REPOSITORIES: Pride

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The Mechano-Ubiquitinome of Articular Cartilage: Differential Ubiquitination and Activation of a Group of ER-Associated DUBs and ER Stress Regulators.

Kaokhum Nitchakarn N Pinto-Fernández Adán A Wilkinson Mark M Kessler Benedikt M BM Ismail Heba M HM

Molecular & cellular proteomics : MCP 20220928 12

Understanding how connective tissue cells respond to mechanical stimulation is important to human health and disease processes in musculoskeletal diseases. Injury to articular cartilage is a key risk factor in predisposition to tissue damage and degenerative osteoarthritis. Recently, we have discovered that mechanical injury to connective tissues including murine and porcine articular cartilage causes a significant increase in lysine-63 polyubiquitination. Here, we identified the ubiquitin signa ...[more]

PMID: 36182100

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