Secretomics of human pluripotent stem cell derived neural progenitor cells and induced astrocytes.

ABSTRACT: This study examines and compares the protein content in conditioned media collected from neural cell types generated from human pluripotent stem cells. Conditioned media was prepared for 48 hours at a final endpoint of differentiation day 12. Both groups are from parental line WTC11 and cultured as a monolayer on matrigel. Both groups contain a transgene cassette for doxycycline-inducible expression of sox9 and nfia. Doxycycline was only included in the iAstro groups, whereas it was omitted in the neural progenitor cell groups.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Mucinous Pancreas Adenocarcinoma

SUBMITTER: Nelufa Islam  

LAB HEAD: Robert C. Krencik

PROVIDER: PXD030821 | Pride | 2022-01-19

REPOSITORIES: Pride