Identification and characterization of β-ODAP synthase in grass pea

ABSTRACT: Grass pea seeds and seedlings protein extracts were chromatographically fractionated. To identify the β-ODAP synthase enzyme, active fractions, as determined by a colorimetric assay that detects the presence of a derivative of free L-α,β-diaminopropionic acid (L-DAPA), were subjected to tryptic digestion and LC-MS/MS and searched against a database containing translated sequences from a long-read PacBio mRNA sequencing of grass pea seeds and seedlings.

INSTRUMENT(S):

ORGANISM(S): Lathyrus Sativus

TISSUE(S): Seedling, Seed

SUBMITTER: Alon Savidor  

LAB HEAD: Ziv Reich

PROVIDER: PXD030847 | Pride | 2022-05-19

REPOSITORIES: Pride