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Designing workflows for large-scale Omic Based biomarker identification for hepatocellular carcinoma

ABSTRACT: Ter-polymer (MMA/EGDMA/1,2-Epoxy-5-Hexene) is fabricated via the polymerization using ethylene glycol di-methacrylate (EGDMA) as polar crosslinker. The oxirane groups of ter-polymer are immobilized with cysteic acid to enhance the hydrophilicity of HILIC sorbent. The enrichment efficacy is evaluated using the tryptic digests of HRP and IgG. Cya@terpolymer shows good potential for the intact N-linked glycopeptides enrichment from human serum.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Habtom Ressom

LAB HEAD: Habtom Ressom

PROVIDER: PXD030890 | Pride | 2022-10-14

REPOSITORIES: Pride

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			Action	DRS
	MALDI_MS_Data.zip	Other
	NanoLCMS_Data.zip	Other
	UP000005640_9606__1_.fasta	Fasta
	pGlycoDB-GP-FDR-Pro.txt	Txt

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Publications

Terpolymeric platform with enhanced hydrophilicity via cysteic acid for serum intact glycopeptide analysis.

Sajid Muhammad Salman MS Saleem Shafaq S Jabeen Fahmida F Najam-Ul-Haq Muhammad M Ressom Habtom W HW

Mikrochimica acta 20220713 8

A new polymeric (methyl methacrylate/ethylene glycol dimethacrylate/1,2-epoxy-5-hexene) base/matrix has been fabricated and decorated with zwitterionic hydrophilic cysteic acid (Cya) for the enrichment of intact N-glycopeptides from standards and biological samples. Terpolymer-Cya provides good enrichment efficiency, improved hydrophilicity, and selectivity by virtue of better surface area (2.09 × 10<sup>2</sup> m<sup>2</sup>/g) provided by terpolymer and the zwitterionic property offered by cys ...[more]

PMID: 35829791

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