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Impact of simutaneous exposure to cold and metals on Salix viminalis transcriptome, proteome and antioxidative machinery

ABSTRACT: Frost is a major abiotic stress limiting plant growth and development in many parts of the world, especially under temperate and cold climates. To withstand freezing stress, plants have evolved an adaptative process known as cold acclimation. With climate changes, models predict an increase in the magnitude and frequency of late-frost events, which, together with an observed loss of soil insulation, will significantly damage roots leading to a decrease in plants primary productivity. While the cold acclimation process is well documented, it is known that plant response to multiple stresses is unique and cannot be deduced from the response to each stress taken separately. Here, we investigate the impact of long-term metal exposure on the cold acclimation of S. viminalis. To do so, we used physiological, transcriptomic and proteomic approaches. We found that while metal exposure significantly affected plants morphology and physiology, it did not impede cold acclimation. The impact of the simultaneous exposure to metals and cold acclimation on the transcriptome was unique. However, cold acclimation seemed to impact more the roots than metals exposure at the proteome level. Further analysis revealed that metals strongly and negatively impacted the cellular antioxidant system. This negative impact was not compensated in plants subsequently cold-acclimated. While this should have led to a loss of frost tolerance, it was not observed. Therefore, we propose a group of proteins that could have played a role in compensating the impediment or the antioxidative system.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Salix Viminalis

TISSUE(S): Plant Cell, Root

SUBMITTER: Céline LECLERCQ

LAB HEAD: Celine LECLERCQ

PROVIDER: PXD030968 | Pride | 2024-01-26

REPOSITORIES: Pride

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Project description:The aim of the experiment is to study the pattern of genes expression involved in defense to heavy metals and their transport and distribution within plant organs. Most of the plant species inhibits heavy metals entrance to the roots and their transport and accumulation to/in above ground organs after toxic ions penetrate roots. Some species of Brassicacea differ in the pattern of defense gene expression in the roots in a way that allow for greater transport of toxic ions to the above ground part. If so, by harvesting such plants toxic elements will be extracted from the soil. Identification of these differences in the gene expression is crucial in development of the newly emerging environmental biotechnology phytoremediation in which plants as well as being green lungs play also a role of a green liver. Results of our studies with mustard plants showed among others that exposure for 12 hr to 12.5mg of Pb/1L of hydroponics solution is efficient to increase of constitutive genes expression and in de novo induction of genes expression coding for Pb tolerance and that this response is organ specific. Sequencing of the whole genome of A. thaliana opens the opportunity to study this phenomenon at molecular level more widely including the signaling (two-component system) and plasmolemma and tonoplast transporters genes. Therefore in our further steps we are going to use Arabidopsis as a model plant and the transcriptomics aproach is the only way for such studies. At first based on results with mustard physiological study we will conduct for elucidating Arabidopsis plant responses to lead in order to choose the most appropriate dose and time of treatment. In the trascriptome experiment we would like to study the profile gene expression in above ground parts and in the roots of arabidopsis plants in response to toxic ions of lead. Results of this study, besides promising applicable aspects, will also lead to a better understanding of plant acclimation and adaptation to antropogenic stresses. We expect to obtain mutant(s) tolerant to Pb and if so, they will be provided to NASC. Experiment Overall Design: 6 samples

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Impact of simutaneous exposure to cold and metals on Salix viminalis transcriptome, proteome and antioxidative machinery

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